ROTI®Transform
Sterile products
Ready-to-use products and reagents
Storage temp. -20 °C
Transport temp. ambient temp.
WGK 1
One kit is sufficient to produce competent E. coli for 500 transformations.
Contents of this Kit may not be bought separately.
€71.50/Pack Qty.
excl. VAT. | 1 kit per Pack Qty.
Art. No. P043.1
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Product details
ROTI®Transform is a simple, effective and fast method of producing competent E. coli in three steps. The competent cells may be used directly for transformation or may be frozen for later transformations.
- Flexible selection of strains
- Effective, simple and fast
- Adequate for 500 transformations
Transformation efficiency is 5 x 105 to 1 x 106 colonies per µg DNA.
ROTI®Transform sterile, ready-to-use
Protocol:
1. Resuspend cells in fresh, cold LB-medium
2. Add ROTI®Transform 1
3. Add ROTI®Transform 2 and incubate for 5 minutes on ice.
ROTI®Transform consists of 45 ml ROTI®Transform 1 (Art. No. P041) and 5 ml ROTI®Transform 2 (Art. No. P042).
Long-term Storage of the Kit at -20 °C.
Storage for use:
Reagent 1: Short-term storage may be done at 4 °C. For long-term storage of over 14 days we recommend a storage temperature of -20 °C.
Reagent 2: Storage at 4 °C.
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| Order No. | Pack Qty. | Pack. | Packaging | Price | Quantity | |
|---|---|---|---|---|---|---|
| P043.1 | 1 kit | cardboard | for approx. 500 transformations |
€71.50 |
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Delivery date currently unknown
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- Subtotal: 0.00