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ROTI®Garose-Biotin Beads, 10 ml

ROTI®Garose for biochemistry
Examples of effect: Flammable; liquids form compounds with the air which can become explosive; produce flammable gases with water or can self-combust. Safety: Keep away from open flames and sources of heat; close containers tightly; store in a fire-safe manner.
Examples of effect: Lead to damage to heath, cause irritation to eyes, skin or respiratory organs. Are fatal in larger quantities. Safety: As previously stated, in the event of skin irritation or contact with eyes, rinse with water or a suitable medium.
Warning
H226-H319
i flammable liquid and vapour, causes serious eye irritation
P210 P280 P305+P351+P338
i keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking, wear protective gloves/eye protection, IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing
Pack Qty.
Pack.
Agarose beads for affinity chromatography, Biotin-Agarose Beads, Column matrix for protein isolation
Density (D) 1 g/cm³
Flash point (flp) 35 °C
Melting point (mp) -114 °C
Storage temp. +4 °C
ADR 3 III
WGK 3
UN-Nr. 1170

For isolation of avidin/streptavidin-coupled molecules by affinity chromatography
For batch mode and gravity flow

Biotin coupled agarose beads for affinity chromatography. 50 % bead suspension in water, stabilised with 0,02 % sodium azide.

ROTI®Garose-Biotin Beads offer the perfect solution for your specific application of low flow column chromatography or isolation in batch mode.
Product details
Type analysis

€815.95/Pack Qty. 

excl. VAT. | 10 ml per Pack Qty.

Art. No. 0844.2

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from 24 Pack Qty. €734.36/Pack Qty.
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Product details



ROTI®Garose-Biotin Beads ROTI®Garose for biochemistry

Biotin coupled agarose beads for affinity chromatography. 50 % bead suspension in water, stabilised with 0,02 % sodium azide.

ROTI®Garose-Biotin Beads offer the perfect solution for your specific application of low flow column chromatography or isolation in batch mode.

  • One-step isolation of avidin/streptavidin-tagged molecules from total lysates
  • Rapid, mild and highly specific purification
  • Suitable for isolation of DNA, proteins and other molecules of all sizes
  • High binding capacity
  • High recovery rates
  • For batch mode and gravity flow

Suitable for

Recommended for isolation of avidin- or streptavidin labelled molecules in batch mode or via columns via low pressure affinity chromatography.


Directions for use

The matrix of ROTI®Garose-Biotin Beads consists of beaded 4 % cross-linked agarose. The matrix is easy to pack and results in very evenly packed columns. Elution is carried out via 8 M guanidine-HCl or prior to gel loading during heating in gel loading buffer.
With a value of Ka~10-14/M, the affinity of avidin and streptavidin for biotin is the strongest non-covalent bond between molecules known in biochemistry. Efficient and highly specific isolation of avidin/streptavidin-tagged molecules via affinity chromatography is based on this particular affinity.
Biotin is immobilised to the beads through a spacer arm and covalent carboxy/amide linkage, not only minimizing biotin leakage during elution, but also enhancing the binding capacity by reduction of steric effects. Merely no unspecific binding of unlabelled molecules takes place, even during purification from raw extracts.
Suspension in water stabilised with 0,02 % sodium azide.



ROTI®Garose-Biotin Beads
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0844.1 5 ml plastic €456.90
0844.2 10 ml plastic €815.95
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Certificates of Analysis

You can search for and download your certificate of analysis for the selected product here. Please provide your batch number.
The following analysis certificates have been found:

Type analysis

Particle size
Beads (cross-linked agarose)4 %  
LigandBiotin  
Binding capacity (gel) (Avidin)≥ 30 mg/ml  
Sodium azide0,02 %  

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