ROTI®Garose-Biotin Beads, 10 ml
Flash point (flp) 35 °C
Melting point (mp) -114 °C
Storage temp. +4 °C
ADR 3 III
WGK 3
UN-Nr. 1170
For batch mode and gravity flow
ROTI®Garose-Biotin Beads offer the perfect solution for your specific application of low flow column chromatography or isolation in batch mode.
€815.95/Pack Qty.
excl. VAT. | 10 ml per Pack Qty.
Art. No. 0844.2
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Product details
ROTI®Garose-Biotin Beads ROTI®Garose for biochemistry
Biotin coupled agarose beads for affinity chromatography. 50 % bead suspension in water, stabilised with 0,02 % sodium azide.
ROTI®Garose-Biotin Beads offer the perfect solution for your specific application of low flow column chromatography or isolation in batch mode.
- One-step isolation of avidin/streptavidin-tagged molecules from total lysates
- Rapid, mild and highly specific purification
- Suitable for isolation of DNA, proteins and other molecules of all sizes
- High binding capacity
- High recovery rates
- For batch mode and gravity flow
Recommended for isolation of avidin- or streptavidin labelled molecules in batch mode or via columns via low pressure affinity chromatography.
The matrix of ROTI®Garose-Biotin Beads consists of beaded 4 % cross-linked agarose. The matrix is easy to pack and results in very evenly packed columns. Elution is carried out via 8 M guanidine-HCl or prior to gel loading during heating in gel loading buffer.
With a value of Ka~10-14/M, the affinity of avidin and streptavidin for biotin is the strongest non-covalent bond between molecules known in biochemistry. Efficient and highly specific isolation of avidin/streptavidin-tagged molecules via affinity chromatography is based on this particular affinity.
Biotin is immobilised to the beads through a spacer arm and covalent carboxy/amide linkage, not only minimizing biotin leakage during elution, but also enhancing the binding capacity by reduction of steric effects. Merely no unspecific binding of unlabelled molecules takes place, even during purification from raw extracts.
Suspension in water stabilised with 0,02 % sodium azide.
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| Order No. | Pack Qty. | Pack. | Price | Quantity | ||
|---|---|---|---|---|---|---|
| 0844.1 | 5 ml | plastic | €456.90 |
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| 0844.2 | 10 ml | plastic | €815.95 |
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- Subtotal: 0.00
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Certificates of Analysis
Type analysis
| Particle size | |
| Beads (cross-linked agarose) | 4 % |
| Ligand | Biotin |
| Binding capacity (gel) (Avidin) | ≥ 30 mg/ml |
| Sodium azide | 0,02 % |