ROTI®Garose-His/Co Beads, 100 ml
Melting point (mp) -114 °C
Storage temp. +4 °C
ADR 3 III
For batch mode and gravity flow
excl. VAT. | 100 ml per Pack Qty.
Art. No. 1235.2
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ROTI®Garose-His/Co Beads ROTI®Garose for biochemistry
Cobalt charged IDA-agarose beads for low pressure affinity chromatography. Suspension in 20 % ethanol.
Immobilized metal ion affinity chromatography (IMAC) still is the most widely used method to obtain high yield of very pure proteins with reasonable effort. ROTI®Garose-His/Co Beads offer the perfect solution for your specific application of low flow column chromatography or protein isolation in batch mode.
- One-Step purification of His-tagged proteins from total lysates
- Good recovery rate of extremely pure His-tagged proteins
- Binding capacity (purified 6xHis protein) of 135 mg per ml matrix
- Easy elution and regeneration
- Very low Cobalt leaching
- Compatible with denaturing reagents
- For batch mode and gravity flow column chromatogrphy
Perfect choice if extremely pure proteins are required, or if hard-to-separate proteins shall be isolated.
1: Marker, 2: Protein raw extract, 3: 6xHis target protein purified with ROTI®Garose His/Co Beads, 4: 6xHis target protein purified by competitor product.
The matrix of ROTI®Garose His/Co Beads consists of crosslinked and beaded 6 % agarose, IDA-conjugated and charged with divalent Cobalt ions. Cobalt chelates recognize two exposed, vicinal histidines with superior specificity and good affinity, resulting in acceptable to good recovery rates only, but yielding proteins of superior purity. This matrix is, therefore, the perfect choice if extremely pure proteins are required, or if hard-to-separate proteins shall be isolated. ROTI®Garose His/Co beads result in eluates with considerably low metal contamination. The tridentate IDA cross-linker provides easy elution with low amounts of imidazole. Additionally, ROTI®Garose Beads may repeatedly be regenerated, making them very cost-effective.
The Matrix is stable in all commonly used reagents including denaturing reagents (like 8 M urea, 6 M guanidinium hydrochloride, 5 mM DTT).
May be autoclaved at 121 °C for 30 mins.
- Subtotal: 0.00
|Order No.||Pack Qty.||Pack.||Price||Quantity|
Available at short notice
Delivery time on request
|Particle size||50-150 µm|
|Beads (cross-linked agarose)||0.06|
|Binding capacity (DHAK-6xHis)||135 mg/ml|
|Stabilizer||20 % ethanol|