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ROTI®Garose-His/Co Beads, 100 ml

ROTI®Garose for biochemistry
Examples of effect: Flammable; liquids form compounds with the air which can become explosive; produce flammable gases with water or can self-combust. Safety: Keep away from open flames and sources of heat; close containers tightly; store in a fire-safe manner.
Examples of effect: Lead to damage to heath, cause irritation to eyes, skin or respiratory organs. Are fatal in larger quantities. Safety: As previously stated, in the event of skin irritation or contact with eyes, rinse with water or a suitable medium.
Examples of effect: Have an allergenic effect, carcinogenic, mutagenic, toxic to reproduction and cause developmental toxicity (reprotoxic) or damage to organs Safety: You must be well-informed before starting work with these substances; wear protective clothing and gloves, eye protection and mask or breathing protection.
danger
H226-H319-H350-EUH208
i flammable liquid and vapour, causes serious eye irritation, may cause cancer
P202 P210 P280.7 P303+P361+P353 P308+P313
i do not handle until all safety precautions have been read and understood, keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking, wear protective gloves/eye protection, IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water [or shower], IF exposed or concerned: Get medical advice/attention
Pack Qty.
Pack.
Cobalt agarose beads, Agarose beads for affinity chromatography, IMAC matrix/cobalt charged, Column matrix for protein isolation, IMAC beads
Flash point (flp) 35 °C
Melting point (mp) -114 °C
Storage temp. +4 °C
ADR 3 III
WGK 3
UN-Nr. 1170

For isolation of His-tagged proteins by affinity chromatography.
For batch mode and gravity flow

Cobalt charged IDA-agarose beads for low pressure affinity chromatography. Suspension in 20 % e ...
Product details
Type analysis

€772.95/Pack Qty. 

excl. VAT. | 100 ml per Pack Qty.

Art. No. 1235.2

Available at short notice
from 6 Pack Qty. €734.30/Pack Qty.
from 24 Pack Qty. €695.66/Pack Qty.
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Product details



ROTI®Garose-His/Co Beads ROTI®Garose for biochemistry

Cobalt charged IDA-agarose beads for low pressure affinity chromatography. Suspension in 20 % ethanol.

Immobilized metal ion affinity chromatography (IMAC) still is the most widely used method to obtain high yield of very pure proteins with reasonable effort. ROTI®Garose-His/Co Beads offer the perfect solution for your specific application of low flow column chromatography or protein isolation in batch mode.

  • One-Step purification of His-tagged proteins from total lysates
  • Good recovery rate of extremely pure His-tagged proteins
  • Binding capacity (purified 6xHis protein) of 135 mg per ml matrix
  • Easy elution and regeneration
  • Very low Cobalt leaching
  • Compatible with denaturing reagents
  • For batch mode and gravity flow column chromatogrphy

Suitable for

Perfect choice if extremely pure proteins are required, or if hard-to-separate proteins shall be isolated.

1235_02
Figure: SDS-PAGE of highly expressed 6xHis-glutaryl acylase, 58 kDa (arrows). Good yield of extremely pure proteins.
1: Marker, 2: Protein raw extract, 3: 6xHis target protein purified with ROTI®Garose His/Co Beads, 4: 6xHis target protein purified by competitor product.

Directions for use

The matrix of ROTI®Garose His/Co Beads consists of crosslinked and beaded 6 % agarose, IDA-conjugated and charged with divalent Cobalt ions. Cobalt chelates recognize two exposed, vicinal histidines with superior specificity and good affinity, resulting in acceptable to good recovery rates only, but yielding proteins of superior purity. This matrix is, therefore, the perfect choice if extremely pure proteins are required, or if hard-to-separate proteins shall be isolated. ROTI®Garose His/Co beads result in eluates with considerably low metal contamination. The tridentate IDA cross-linker provides easy elution with low amounts of imidazole. Additionally, ROTI®Garose Beads may repeatedly be regenerated, making them very cost-effective.

The Matrix is stable in all commonly used reagents including denaturing reagents (like 8 M urea, 6 M guanidinium hydrochloride, 5 mM DTT).
May be autoclaved at 121 °C for 30 mins.



ROTI®Garose-His/Co Beads
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1235.1 25 ml plastic €284.90
1235.2 100 ml plastic €772.95
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Downloads


Analysis certificates

You can search for and download your certificate of analysis for the selected product here. Please provide your batch number.
The following analysis certificates have been found:

Type analysis

Particle size50-150 µm
Beads (cross-linked agarose)0.06
LigandIDA
Metal ionCobalt
Binding capacity (DHAK-6xHis)135 mg/ml
Activating agentEpichlorohydrin
Stabilizer20 % ethanol

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