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ROTI®Garose-His/Co Columns, 5 unit(s), 5 × 5 ml

ROTI®Garose for biochemistry
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Danger
H226-H319-H350-H411-EUH208
i flammable liquid and vapour, causes serious eye irritation, may cause cancer, toxic to aquatic life with long lasting effects
P202 P210 P273 P280 P303+P361+P353 P308+P313
i do not handle until all safety precautions have been read and understood, keep away from heat. No smoking, avoid release to the environment, wear protective gloves/eye protection, IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water, IF exposed or concerned: Get medical advice/attention
Pack Qty.
Pack.
Packaging
Cobalt agarose beads, Cobalt charged IMAC columns, IMAC columns, Affinity chromatography colums
Density (D) 0,95 g/cm³
Boiling point (bp) >80 °C
Flash point (flp) >50 °C
Storage temp. +4 °C
ADR 3 III
WGK 3
UN-Nr. 1170

For isolation of His-tagged proteins by affinity chromatography.
Prefilled colums for gravity flow chromatography.
Matrix volume 1 or 5 ml, whole volume 12 or 35 ml, respectively.
For approx. 120 and 600 mg His labelled protein.

Prepacked columns with Cobalt charged IDA-agarose bead matrix for low pressure affinity chromatography.

Immobilized metal ion affinity chromatography (IMAC) still is the most widely used method to obtain high yield of very pure proteins with reasonable effort. ROTI®Garose His/Co Columns offer the perfect solution for fast and simple isolation of His-tagged proteins from total lysates.
Product details

€736.40/Pack Qty. 

excl. VAT. | 5 unit(s) per Pack Qty.

Art. No. 1267.2

Available at short notice
from 6 Pack Qty. €699.58/Pack Qty.
from 24 Pack Qty. €662.76/Pack Qty.
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Product details



ROTI®Garose-His/Co Columns ROTI®Garose for biochemistry

Prepacked columns with Cobalt charged IDA-agarose bead matrix for low pressure affinity chromatography.

Immobilized metal ion affinity chromatography (IMAC) still is the most widely used method to obtain high yield of very pure proteins with reasonable effort. ROTI®Garose His/Co Columns offer the perfect solution for fast and simple isolation of His-tagged proteins from total lysates.

  • Fast and simple purification of His-tagged proteins from total lysates
  • Good recovery rate of extremely pure His-tagged proteins
  • Binding capacity (purified 6xHis protein) of 135 mg per ml matrix
  • Easy elution and regeneration
  • Very low Cobalt leaching
  • Compatible with denaturing reagents
  • For gravity flow chromatography

Perfect choice if extremely pure proteins are required, or if hard-to-separate proteins shall be isolated.


ROTI®Garose His/Co Columns are prepacked and may be used directly. The matrix consists of cross-linked and beaded 6 % agarose, IDA-conjugated and charged with divalent Cobalt ions. Cobalt chelates recognize two exposed, vicinal histidines with superior specificity and good affinity, resulting in acceptable to good recovery rates only, but yielding proteins of superior purity. This matrix is, therefore, the perfect choice if extremely pure proteins are required, or if hard-to-separate proteins shall be isolated. ROTI®Garose His/Co Columns result in eluates with considerably low metal contamination. The tridentate IDA cross-linker provides easy elution with low amounts of imidazole. Additionally, ROTI®Garose Columns may repeatedly be regenerated, making them very cost-effective.

The Matrix is stable in all commonly used reagents including denaturing reagents (like 8 M urea, 6 M guanidinium hydrochloride, 5 mM DTT).
Column material made from polypropylene and polyethylene (frit). Suspension in 20 % ethanol.
May be autoclaved at 121 °C for 30 mins.



Technical Information
Instructions for use, addition Prepacked columns, binding capacity ca. 120 (.1) resp. 600 (.2) mg/column (His)6 
ROTI®Garose-His/Co Columns
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Order No. Pack Qty. Pack. Packaging Price Quantity
1267.1 8 unit(s) cardboard 8 x 1 ml €284.90
1267.2 5 unit(s) cardboard 5 × 5 ml €736.40
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Downloads / MSDS


Certificates of Analysis

You can search for and download your certificate of analysis for the selected product here. Please provide your batch number.
The following analysis certificates have been found:

Type analysis

Particle size50-150 µm
Beads (cross-linked agarose)6 %
LigandIDA
Metal ionCobalt
Binding capacity (DHAK-6xHis)135 mg/ml
Activating agentEpichlorohydrin
Stabilizer20 % ethanol