Technical Data Sheet
ROTI®Garose-His/Co HPBeads, 25 ml
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Boiling point (bp) >80 °C
Flash point (flp) >50 °C
Storage temp. +4 °C
ADR 3 III
WGK 3
UN-Nr. 1170
Suitable for LPLC, FPLC and MPLC and big matrix volumes
Immobilized metal ion affinity chromatography (IMAC) still is the most widely used method to obtain high yield of very pure proteins with reasonable effort.
ROTI®Garose His/Co Beads and ready-to-use columns offer the perfect solution for your specific applications in the field of benchtop column chromatography, for protein isolation in batch processes or under medium pressures (MPLC, FPLC). Depending on the product, our beads are optimally suited for low or high flow rates or when small or large sample volumes are to be purified. The ready-to-use columns and cartridges offer you the perfect solution for fast and easy purification of His-tag proteins from total lysate.
€370.90/Pack Qty.
excl. VAT. | 25 ml per Pack Qty.
Art. No. 0838.1
Fits to / Accessories
Product details
For the isolation of high yields of highly pure His-tagged proteins under non-reducing conditions.
Immobilized metal ion affinity chromatography (IMAC) still is the most widely used method to obtain high yield of very pure proteins with reasonable effort.
ROTI®Garose His/Co Beads and ready-to-use columns offer the perfect solution for your specific applications in the field of benchtop column chromatography, for protein isolation in batch processes or under medium pressures (MPLC, FPLC). Depending on the product, our beads are optimally suited for low or high flow rates or when small or large sample volumes are to be purified. The ready-to-use columns and cartridges offer you the perfect solution for fast and easy purification of His-tag proteins from total lysate.
- Good recovery rate of extremely pure His-tagged proteins
- Very low Cobalt leaching
- Compatible with denaturing reagents
The matrix of ROTI®Garose His/Co products consists of crosslinked and beaded 6 % agarose, IDA-conjugated and charged with divalent Cobalt ions. Cobalt chelates recognize two histidines consecutive in an amino acid sequence with superior specificity and good affinity, resulting in acceptable to good recovery rates only, but yielding proteins of superior purity. This matrix is, therefore, the perfect choice if extremely pure proteins are required, or if hard-to-separate proteins shall be isolated. ROTI®Garose His/Co beads result in eluates with considerably low metal contamination.
The tridentate IDA cross-linker provides easy elution with low amounts of imidazole. Additionally, ROTI®Garose Beads may repeatedly be regenerated, making them very cost-effective.
The Matrix is stable in all commonly used reagents including denaturing reagents (like 8 M urea, 6 M guanidinium hydrochloride, 5 mM DTT).
For insulation under reducing conditions we recommend ROTI®Garose-His/Ni NTA beads.
ROTI®Garose-His/Co HPBeads ROTI®Garose for biochemistry
Nickel charged IDA-agarose beads for affinity chromatography under medium pressure, with high flow rate or with big sample-/matrix volume.
In ROTI®Garose-His/Co HPBeads, the advantages of the highly efficient cobalt-His binding have been combined with a bead technology allowing a very rapid flow rate. ROTI®Garose-His/Co HPBeads are ideal for purifying large sample volumes.
50 % bead suspension in 20 % ethanol.
- Fast one-step purification of highly purified His-tag proteins from total lysate
- High binding capacity and excellent specificity for 6xHis-tagged protein
- Easy elution and regeneration
- For batch mode, gravity flow, MPLC und FPLC
- Recommended for big matrix volumes
MPLC and FPLC, or if superiorly pure His-tag proteins shall be isolated either in particularly short time or in big amounts.
May be autoclaved at 121 °C for 30 mins.
| Instructions for use, addition | Bead suspension |
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| Art. No. | Pack Qty. | Pack. | Price | Quantity | |
|---|---|---|---|---|---|
| 0838.1 | 25 ml | plastic |
€370.90 |
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| 0838.2 | 100 ml | plastic |
€1,009.45 |
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In stock
Available
In procurement
No longer available
Delivery date currently unknown
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- Subtotal: 0.00
Downloads / MSDS
General information
Further attractive products to complete your chromatography laboratory can be found on our Chromatography page!
Certificates of Analysis
Type analysis
| Particle size | 50-150 µm |
| Beads (cross-linked agarose) | 6 % |
| Ligand | IDA |
| Metal ion | cobalt |
| Loading capacity (gel) (Co2+) | ~20 µmol/ml |
| Size exclusion limit (Da) | ~4x10^6 |
| Ethanol | 20 % |