ROTI®Garose-His/Ni Beads, 100 ml
Melting point (mp) -114 °C
Storage temp. +4 °C
ADR 3 III
For batch mode and gravity flow
excl. VAT. | 100 ml per Pack Qty.
Art. No. 1308.2
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ROTI®Garose-His/Ni Beads ROTI®Garose for biochemistry
Nickel charged IDA-agarose beads for low pressure affinity chromatography. Suspension in 20 % ethanol.
Immobilized metal ion affinity chromatography (IMAC) still is the most widely used method to obtain high yield of very pure proteins with reasonable effort. ROTI®Garose His/Ni Beads offer the perfect solution for your specific application of low flow column chromatography or protein isolation in batch mode.
- One-Step purification of His-tagged proteins from total lysates
- Superior recovery rate of pure His-tagged proteins
- Binding capacity (purified 6xHis protein) of 117 mg per ml matrix
- Easy elution and regeneration
- Very low Nickel leaching
- Compatible with denaturing reagents
- For batch mode and gravity flow column chromatography
Suitable for all general applications, or if proteins of very small amount shall be isolated without loss.
1: Marker, 2: Protein raw extract, 3,4: 6xHis target protein purified with ROTI®Garose His/Ni Beads or Column, respectively, 5: 6xHis target protein purified by competitor product.
The matrix of ROTI®Garose His/Ni Beads consists of crosslinked and beaded 6 % agarose, IDA-conjugated and charged with divalent Nickel ions. Nickel chelates recognize two exposed histidines with good specificity and very high affinity, making the Ni2+ charged matrix the first choice for all standard applications. ROTI®Garose His/Ni beads give very high yields of pure His-tagged protein with considerably low metal contamination. The tridentate IDA cross-linker provides easy elution with low amounts of imidazole. Additionally, ROTI®Garose Beads may repeatedly be regenerated, making them very cost-effective.
The Matrix is stable in all commonly used reagents including denaturing reagents (like 8 M urea, 6 M guanidinium hydrochloride, 5 mM DTT). May be autoclaved at 121 °C for 30 mins.
- Subtotal: 0.00
|Order No.||Pack Qty.||Pack.||Price||Quantity|
Available at short notice
Delivery time on request
|Particle size||50-150 µm|
|Beads (cross-linked agarose)||0.06|
|Binding capacity (DHAK-6xHis)||117 mg/ml|
|Stabilizer||20 % ethanol|