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ROTI®Garose-His/Ni Beads, 100 ml

ROTI®Garose for biochemistry
Examples of effect: Flammable; liquids form compounds with the air which can become explosive; produce flammable gases with water or can self-combust. Safety: Keep away from open flames and sources of heat; close containers tightly; store in a fire-safe manner.
Examples of effect: Lead to damage to heath, cause irritation to eyes, skin or respiratory organs. Are fatal in larger quantities. Safety: As previously stated, in the event of skin irritation or contact with eyes, rinse with water or a suitable medium.
i flammable liquid and vapour, causes serious eye irritation
P210 P280 P303+P361+P353 P305+P351+P338
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Nickel agarose beads, Agarose beads for affinity chromatography, IMAC matrix/nickel charged, Column matrix for protein isolation, IMAC beads
Flash point (flp) 35 °C
Melting point (mp) -114 °C
Storage temp. +4 °C
UN-Nr. 1170

For isolation of His-tagged proteins by affinity chromatography.
For batch mode and gravity flow

Nickel charged IDA-agarose beads for low pressure affinity chromatography. Suspension in 20 % e ...
Product details
Type analysis

€805.20/Pack Qty. 

excl. VAT. | 100 ml per Pack Qty.

Art. No. 1308.2

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from 6 Pack Qty. €764.94/Pack Qty.
from 24 Pack Qty. €724.68/Pack Qty.
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Product details

ROTI®Garose-His/Ni Beads ROTI®Garose for biochemistry

Nickel charged IDA-agarose beads for low pressure affinity chromatography. Suspension in 20 % ethanol.

Immobilized metal ion affinity chromatography (IMAC) still is the most widely used method to obtain high yield of very pure proteins with reasonable effort. ROTI®Garose His/Ni Beads offer the perfect solution for your specific application of low flow column chromatography or protein isolation in batch mode.

  • One-Step purification of His-tagged proteins from total lysates
  • Superior recovery rate of pure His-tagged proteins
  • Binding capacity (purified 6xHis protein) of 117 mg per ml matrix
  • Easy elution and regeneration
  • Very low Nickel leaching
  • Compatible with denaturing reagents
  • For batch mode and gravity flow column chromatography

Suitable for

Suitable for all general applications, or if proteins of very small amount shall be isolated without loss.

Directions for use

The matrix of ROTI®Garose His/Ni Beads consists of crosslinked and beaded 6 % agarose, IDA-conjugated and charged with divalent Nickel ions. Nickel chelates recognize two exposed histidines with good specificity and very high affinity, making the Ni2+ charged matrix the first choice for all standard applications. ROTI®Garose His/Ni beads give very high yields of pure His-tagged protein with considerably low metal contamination. The tridentate IDA cross-linker provides easy elution with low amounts of imidazole. Additionally, ROTI®Garose Beads may repeatedly be regenerated, making them very cost-effective.

The Matrix is stable in all commonly used reagents including denaturing reagents (like 8 M urea, 6 M guanidinium hydrochloride, 5 mM DTT). May be autoclaved at 121 °C for 30 mins.

ROTI®Garose-His/Ni Beads
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1308.2 100 ml plastic €805.20
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Certificates of Analysis

You can search for and download your certificate of analysis for the selected product here. Please provide your batch number.
The following analysis certificates have been found:

Type analysis

Particle size50-150 µm  
Beads (cross-linked agarose)6 %  
Metal ionnickel  
Binding capacity (DHAK-6xHis)117 mg/ml  
Activating agentEpichlorohydrin  
Stabilizer20 % ethanol  

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