Technical Data Sheet
ROTI®Garose-His/Ni Columns, 5 unit(s), 5 x 5 ml
Ready-to-use products and reagents
Boiling point (bp) >80 °C
Flash point (flp) >50 °C
Storage temp. +4 °C
ADR 3 III
WGK 3
UN-Nr. 1170
Prepacked colums for gravity flow chromatography.
Matrix volume 1 or 5 ml, whole volume 12 or 35 ml, respectively.
For approx. 100 and 500 mg His labelled protein.
Immobilized metal ion affinity chromatography (IMAC) still is the most widely used method to obtain high yield of very pure proteins with reasonable effort.
ROTI®Garose His/Ni Beads and ready-to-use columns offer the perfect solution for your specific applications in the field of benchtop column chromatography, for protein isolation in batch processes or under medium pressures (MPLC, FPLC). Depending on the product, our beads are optimally suited for low or high flow rates or when small or large sample volumes are to be purified. The ready-to-use columns and cartridges offer you the perfect solution for fast and easy purification of His-tag proteins from total lysate.
€908.40/Pack Qty.
excl. VAT. | 5 unit(s) per Pack Qty.
Art. No. 1314.2
Fits to / Accessories
Product details
For the isolation of very high yields of pure His-tagged proteins under non-reducing conditions.
Immobilized metal ion affinity chromatography (IMAC) still is the most widely used method to obtain high yield of very pure proteins with reasonable effort.
ROTI®Garose His/Ni Beads and ready-to-use columns offer the perfect solution for your specific applications in the field of benchtop column chromatography, for protein isolation in batch processes or under medium pressures (MPLC, FPLC). Depending on the product, our beads are optimally suited for low or high flow rates or when small or large sample volumes are to be purified. The ready-to-use columns and cartridges offer you the perfect solution for fast and easy purification of His-tag proteins from total lysate.
- Superior recovery rate of pure His-tagged proteins
- Very low Nickel leaching
- Compatible with denaturing reagents
The matrix of ROTI®Garose His/Ni products consists of crosslinked and beaded 6 % agarose, IDA-conjugated and charged with divalent Nickel ions. Nickel chelates recognize two exposed histidines with good specificity and very high affinity, making the Ni2+ charged matrix the first choice for all standard applications. ROTI®Garose His/Ni beads give very high yields of pure His-tagged protein with considerably low metal contamination.
The tridentate IDA cross-linker provides easy elution with low amounts of imidazole. Additionally, ROTI®Garose Beads may repeatedly be regenerated, making them very cost-effective.
The Matrix is stable in all commonly used reagents including denaturing reagents (like 8 M urea, 6 M guanidinium hydrochloride, 5 mM DTT).
A special feature is the modified polychelate linker used in the ROTI®Garose-His/Ni HPBeads plus (0806). For more detailed data please see this product.
For insulation under reducing conditions we recommend ROTI®Garose-His/Ni HPBeads plus or ROTI®Garose-His/Ni NTA beads.
ROTI®Garose-His/Ni Columns ROTI®Garose for biochemistry
Prepacked columns with nickel-loaded IDA agarose bead matrix for low-pressure affinity chromatography.
ROTI®Garose His/Ni Columns offer the perfect solution for fast and simple isolation of His-tagged proteins from total lysates.
Matrix in 20 % ethanol.
- Fast and simple purification of His-tagged proteins from total lysates
- Binding capacity (purified 6xHis protein) of 117 mg per ml matrix
- Easy elution and regeneration
- For gravity flow chromatography
all general applications, or if proteins of very small amount shall be isolated without loss.
Column material made from polypropylene and polyethylene (frit).
May be autoclaved at 121 °C for 30 mins.
| Instructions for use, addition | Prepacked columns, binding capacity ca. 100 (.1) resp. 500 (.2) mg/column (His)6 |
- Subtotal: 0.00
| Art. No. | Pack Qty. | Pack. | Packaging | Price | Quantity | |
|---|---|---|---|---|---|---|
| 1314.1 | 8 unit(s) | cardboard | 8 x 1 ml |
€349.40 |
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| 1314.2 | 5 unit(s) | cardboard | 5 x 5 ml |
€908.40 |
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In stock
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No longer available
Delivery date currently unknown
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- Subtotal: 0.00
Downloads / MSDS
General information
Further attractive products to complete your chromatography laboratory can be found on our Chromatography page!
Certificates of Analysis
Type analysis
| Particle size | 50-150 µm |
| Beads (cross-linked agarose) | 6 % |
| Ligand | IDA |
| Metal ion | nickel |
| Binding capacity (DHAK-6xHis) | 117 mg/ml |
| Stabilizer | 20 % ethanol |