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ROTI®Garose-His/Ni HPBeads, 100 ml

ROTI®Garose for biochemistry
Examples of effect: Flammable; liquids form compounds with the air which can become explosive; produce flammable gases with water or can self-combust. Safety: Keep away from open flames and sources of heat; close containers tightly; store in a fire-safe manner.
Examples of effect: Lead to damage to heath, cause irritation to eyes, skin or respiratory organs. Are fatal in larger quantities. Safety: As previously stated, in the event of skin irritation or contact with eyes, rinse with water or a suitable medium.
Warning
H226-H319-EUH208
i flammable liquid and vapour, causes serious eye irritation
P210 P280 P303+P361+P353 P305+P351+P338
i keep away from heat. No smoking, wear protective gloves/eye protection, IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water, IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing
Related products
Pack Qty.
Pack.
Nickel agarose beads, Agarose beads for affinity chromatography, IMAC matrix/nickel charged, Column matrix for protein isolation, IMAC beads
Density (D) 0,95 g/cm³
Boiling point (bp) >80 °C
Flash point (flp) >50 °C
Storage temp. +4 °C
ADR 3 III
WGK 3
UN-Nr. 1170

For isolation of His-tagged proteins by fast or large scale affinity chromatography
Suitable for LPLC, FPLC and MPLC and big matrix volumes

For the isolation of very high yields of pure His-tagged proteins under non-reducing conditions.

Immobilized metal ion affinity chromatography (IMAC) still is the most widely used method to obtain high yield of very pure proteins with reasonable effort.
ROTI®Garose His/Ni Beads and ready-to-use columns offer the perfect solution for your specific applications in the field of benchtop column chromatography, for protein isolation in batch processes or under medium pressures (MPLC, FPLC). Depending on the product, our beads are optimally suited for low or high flow rates or when small or large sample volumes are to be purified. The ready-to-use columns and cartridges offer you the perfect solution for fast and easy purification of His-tag proteins from total lysate.
Product details

€983.65/Pack Qty. 

excl. VAT. | 100 ml per Pack Qty.

Art. No. 0835.2

In production
from 6 Pack Qty. €934.47/Pack Qty.
from 24 Pack Qty. €885.28/Pack Qty.
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Product details


For the isolation of very high yields of pure His-tagged proteins under non-reducing conditions.

Immobilized metal ion affinity chromatography (IMAC) still is the most widely used method to obtain high yield of very pure proteins with reasonable effort.
ROTI®Garose His/Ni Beads and ready-to-use columns offer the perfect solution for your specific applications in the field of benchtop column chromatography, for protein isolation in batch processes or under medium pressures (MPLC, FPLC). Depending on the product, our beads are optimally suited for low or high flow rates or when small or large sample volumes are to be purified. The ready-to-use columns and cartridges offer you the perfect solution for fast and easy purification of His-tag proteins from total lysate.

  • Superior recovery rate of pure His-tagged proteins
  • Very low Nickel leaching
  • Compatible with denaturing reagents

The matrix of ROTI®Garose His/Ni products consists of crosslinked and beaded 6 % agarose, IDA-conjugated and charged with divalent Nickel ions. Nickel chelates recognize two exposed histidines with good specificity and very high affinity, making the Ni2+ charged matrix the first choice for all standard applications. ROTI®Garose His/Ni beads give very high yields of pure His-tagged protein with considerably low metal contamination.


Directions for use

The tridentate IDA cross-linker provides easy elution with low amounts of imidazole. Additionally, ROTI®Garose Beads may repeatedly be regenerated, making them very cost-effective.
The Matrix is stable in all commonly used reagents including denaturing reagents (like 8 M urea, 6 M guanidinium hydrochloride, 5 mM DTT).

A special feature is the modified polychelate linker used in the ROTI®Garose-His/Ni HPBeads plus (0806). For more detailed data please see this product.
For insulation under reducing conditions we recommend ROTI®Garose-His/Ni HPBeads plus or ROTI®Garose-His/Ni NTA beads.



ROTI®Garose-His/Ni HPBeads ROTI®Garose for biochemistry

Nickel charged IDA-agarose beads for affinity chromatography under medium pressure, with high flow rate or with big sample-/matrix volume.
In ROTI®Garose-His/Ni HPBeads, the advantages of the highly efficient Nickel-His binding have been combined with a bead technology allowing a very rapid flow rate. ROTI®Garose-His/Ni HPBeads are ideal for purifying large sample volumes.
50 % bead suspension in 20 % ethanol.

  • Rapid one-step purification of very pure His-tagged proteins from total lysates
  • Very high binding capacity for 6xHis-tagged protein
  • Easy elution and regeneration
  • For batch mode, gravity flow, MPLC und FPLC
  • Recommended for big matrix volumes

Suitable for

MPLC and FPLC, or if His-tag proteins shall be isolated either in particularly short time or in big amounts.


Directions for use

May be autoclaved at 121 °C for 30 mins.



Technical Information
Instructions for use, addition Bead suspension 
ROTI®Garose-His/Ni HPBeads
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Art. No. Pack Qty. Pack. Price Quantity
0835.1 25 ml plastic

€360.15

0835.2 100 ml plastic

€983.65

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Downloads / MSDS


General information

Further attractive products to complete your chromatography laboratory can be found on our Chromatography page!


Certificates of Analysis

You can search for and download your certificate of analysis for the selected product here. Please provide your batch number.
The following analysis certificates have been found:

Type analysis

Particle size50-150 µm
Beads (cross-linked agarose)6 %
LigandIDA
Metal ionnickel
Loading capacity (gel) (Ni2+)~20 µmol/ml
Size exclusion limit (Da)~4x106
Ethanol20 %