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ROTI®Garose-His/Ni HPCartridges, 5 unit(s), 5 × 5 ml

ROTI®Garose for biochemistry
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Examples of effect: Flammable; liquids form compounds with the air which can become explosive; produce flammable gases with water or can self-combust. Safety: Keep away from open flames and sources of heat; close containers tightly; store in a fire-safe manner.
Examples of effect: Lead to damage to heath, cause irritation to eyes, skin or respiratory organs. Are fatal in larger quantities. Safety: As previously stated, in the event of skin irritation or contact with eyes, rinse with water or a suitable medium.
Warning
H226-H319-EUH208
i flammable liquid and vapour, causes serious eye irritation
P210 P280 P303+P361+P353 P305+P351+P338
i keep away from heat. No smoking, wear protective gloves/eye protection, IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water, IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing
Related products
Pack Qty.
Pack.
Packaging
IMAC columns, Nickel agarose beads, Nickel charged IMAC columns, Affinity chromatography colums
Density (D) 0,95 g/cm³
Boiling point (bp) >80 °C
Flash point (flp) >50 °C
Storage temp. +4 °C
ADR 3 III
WGK 3
UN-Nr. 1170

For isolation of His-tagged proteins by high pressure affinity chromatography.
Suitable for MPLC, FPLC, ÄKTA™ and peristaltic pumps.
Matrix volume 5 ml, whole volume 10 ml. For approx. 500 mg His labelled protein.

Prepacked cartridges with Nickel charged IDA-agarose bead matrix for medium pressure affinity chromatography (MPLC). Optimal for automated liquid chromatography, or if proteins shall be isolated under pressure. Prepacked matrix, stabilised with 20 % ethanol.
In ROTI®Garose His/Ni HPBeads, the advantages of the highly efficient Nickel-His binding have been combined with a bead technology allowing a very rapid flow rate.

Immobilized metal ion affinity chromatography (IMAC) under medium pressure is the prefect method to obtain high yield of very pure proteins in minimum time. ROTI®Garose His/Ni HPCartridges offer the perfect solution for fast and simple isolation of His-tagged proteins from total lysates.
Product details

€869.70/Pack Qty. 

excl. VAT. | 5 unit(s) per Pack Qty.

Art. No. 1318.2

Available at short notice
from 6 Pack Qty. €826.22/Pack Qty.
from 24 Pack Qty. €782.73/Pack Qty.
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Product details



ROTI®Garose-His/Ni HPCartridges ROTI®Garose for biochemistry

Prepacked cartridges with Nickel charged IDA-agarose bead matrix for medium pressure affinity chromatography (MPLC). Optimal for automated liquid chromatography, or if proteins shall be isolated under pressure. Prepacked matrix, stabilised with 20 % ethanol.
In ROTI®Garose His/Ni HPBeads, the advantages of the highly efficient Nickel-His binding have been combined with a bead technology allowing a very rapid flow rate.

Immobilized metal ion affinity chromatography (IMAC) under medium pressure is the prefect method to obtain high yield of very pure proteins in minimum time. ROTI®Garose His/Ni HPCartridges offer the perfect solution for fast and simple isolation of His-tagged proteins from total lysates.

  • Very fast and simple purification of His-tagged proteins from total lysates
  • Superior recovery rate of pure His-tagged proteins
  • Binding capacity (purified 6xHis protein) of 110 mg per ml matrix / 550 mg per cartridge
  • Recommended flow rate 5 ml/min.
  • Recommended pressure 5 bar [0,5 MPa] (70 psi)
  • Standard ports 10-32 (1/16″)
  • Easy elution and regeneration
  • Very low Nickel leaching
  • Compatible with denaturing reagents
  • Very versatile. Applicable with ÄKTA™FPLC™, pump and syringe.
  • For medium pressure flow chromatography

Suitable for

ÄKTA™FPLC™ and for sample application via peristaltic pump or syringe.

Column material made from polypropylene and polyethylene (frit), Inner diameter 1,6 cm, height 2,5 cm, inlet port 10-32 (1/16″) female, outlet port 10-32 (1/16″) male.


Directions for use

ROTI®Garose His/Ni HPCartridges are prepacked and may be used directly. The matrix consists of cross-linked and beaded 6 % agarose, IDA-conjugated and charged with divalent Nickel ions. Nickel chelates recognize two exposed histidines with good specificity and very high affinity, making the Ni2+ charged matrix the first choice for all standard applications. ROTI®Garose His/Ni HPCartridges give very high yields of pure His-tagged protein with considerably low metal contamination. The tridentate IDA cross-linker provides easy elution with low amounts of imidazole. Additionally, ROTI®Garose His/Ni HPCartridges may repeatedly be regenerated, making them very cost-effective.

The Matrix is stable in all commonly used reagents including denaturing reagents (like 8 M urea, 6 M guanidinium hydrochloride, 5 mM DTT). Suspension in 20 % ethanol.



Technical Information
Instructions for use, addition Prepacked columns, binding capacity ca. 500 mgl/column 
ROTI®Garose-His/Ni HPCartridges
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Order No. Pack Qty. Pack. Packaging Price Quantity
1318.1 1 unit(s) cardboard 1 x 5 ml €205.90
1318.2 5 unit(s) cardboard 5 × 5 ml €869.70
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Downloads / MSDS


Certificates of Analysis

You can search for and download your certificate of analysis for the selected product here. Please provide your batch number.
The following analysis certificates have been found:

Type analysis

Particle size20-50 µm
Beads (cross-linked agarose)6 %
LigandIDA
Metal ionnickel
Binding capacity (DHAK-6xHis)110 mg/ml
Stabilizer20 % ethanol

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