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ROTI®Garose-His/Ni NTA-Cartridges, 1 x 5 ml

ROTI®Garose for biochemistry
Icon_ready-to-use Ready-to-use products and reagents
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Warning
H226-H319-EUH208
i flammable liquid and vapour, causes serious eye irritation
P210 P280 P303+P361+P353 P305+P351+P338
i keep away from heat. No smoking, wear protective gloves/eye protection, IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water, IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing
Pack Qty.
Pack.
Packaging
Nickel agarose beads, Agarose beads for affinity chromatography, IMAC columns
Density (D) 0,95 g/cm³
Boiling point (bp) >80 °C
Flash point (flp) >50 °C
Storage temp. +4 °C
ADR 3 III
WGK 3
UN-Nr. 1170

For isolation of His-tagged proteins by high pressure affinity chromatography under reducing conditions
Suitable for MPLC, FPLC, ÄKTA™ and peristaltic pumps.
Matrix volume 5 ml, whole volume 10 ml. For approx. 250 mg His labelled protein.

For the isolation of His-tagged proteins under reducing conditions.

Immobilized metal ion affinity chromatography (IMAC) still is the most widely used method to obtain high yield of very pure proteins with reasonable effort.
ROTI®Garose His/Ni Beads and ready-to-use columns offer the perfect solution for your specific applications in the field of benchtop column chromatography, for protein isolation in batch processes or under medium pressures (MPLC, FPLC). Depending on the product, our beads are optimally suited for low or high flow rates or when small or large sample volumes are to be purified.
Product details

€246.20/Pack Qty. 

excl. VAT. | 5 ml per Pack Qty.

Art. No. 0804.1

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from 6 Pack Qty. €233.89/Pack Qty.
from 24 Pack Qty. €221.58/Pack Qty.
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Product details


For the isolation of His-tagged proteins under reducing conditions.

Immobilized metal ion affinity chromatography (IMAC) still is the most widely used method to obtain high yield of very pure proteins with reasonable effort.
ROTI®Garose His/Ni Beads and ready-to-use columns offer the perfect solution for your specific applications in the field of benchtop column chromatography, for protein isolation in batch processes or under medium pressures (MPLC, FPLC). Depending on the product, our beads are optimally suited for low or high flow rates or when small or large sample volumes are to be purified.

  • Superior recovery rate of pure His-tagged proteins
  • Minimized Nickel leaching
  • Compatible with denaturing and reducing reagents

The matrix of ROTI®Garose-His/Ni NTA products consists of crosslinked and beaded 6 % agarose, NTA-conjugated and charged with divalent Nickel ions. Nickel chelates recognize two exposed histidine tags with good specificity and very high affinity, making the Ni2+ charged matrix the first choice for all standard applications. The tetradentate NTA cross-linker binds his-tagged proteins very efficiently, leading to high recovery rates with minimized nickel bleeding into the eluate.


Directions for use

ROTI®Garose-His/Ni NTA-Beads may be regenerated, making them very cost-effective.
The Matrix is stable in all commonly used reagents including denaturing reagents (like 8 M urea, 6 M guanidinium hydrochloride) and (dependent on the respective buffer) reducing substances (for instance ≤30 mM glutathion, ≤10  mM DTT, ≤10 mM DTE, ≤20 mM β-mercaptoethanol und ≤0,3 % SDS).



ROTI®Garose-His/Ni NTA-Cartridges ROTI®Garose for biochemistry

Prepacked cartridges with Nickel charged NTA-agarose bead matrix for medium pressure affinity chromatography (MPLC) under reducing conditions.
Immobilized metal ion affinity chromatography (IMAC) under medium pressure is the prefect method to obtain high yield of very pure proteins in minimum time. ROTI®Garose-His/Ni NTA-Cartridges offer the perfect solution for fast and simple isolation of His-tagged proteins from total lysates in reducing buffer solutions.
Matrix in 15 % ethanol.

  • Very fast and simple purification of His-tagged proteins from total lysates
  • High binding capacity for 6×His-tagged protein
  • Recommended flow rate 5 ml/min
  • Standard ports 10-32 (1/16″)
  • Very versatile. Applicable with ÄKTAFPLC, pump and syringe
  • Reliable elution and regeneration
  • For medium pressure flow chromatography

Suitable for

ÄKTA™FPLC™ and for sample application via peristaltic pump or syringe.


Directions for use

ROTI®Garose His/Ni NTA-Cartridges are prepacked and may be used directly.

Column material made from polypropylene and polyethylene (frit), Inner diameter 1,6 cm, height 2,5 cm, inlet port 10-32 (1/16″) female, outlet port 10-32 (1/16″) male.



Technical Information
Instructions for use, addition Prepacked columns, binding capacity 50-250 mg/column (His)6 
ROTI®Garose-His/Ni NTA-Cartridges
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Order No. Pack Qty. Pack. Packaging Price Quantity
0804.1 5 ml plastic 1 x 5 ml

€246.20

0804.2 5 ml plastic 5 x 5 ml

€886.90

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Downloads / MSDS


General information

All SOLVAGREEN® products as well as further information about the product can also be found in our current flyer Green Chemistry - Alternative Solvents and Products.


Further attractive products to complete your chromatography laboratory can be found on our Chromatography page!


Certificates of Analysis

You can search for and download your certificate of analysis for the selected product here. Please provide your batch number.
The following analysis certificates have been found:

Type analysis

Column volume5 ml
Particle size50-150 µm
Beads (cross-linked agarose)6 %
LigandNTA
Metal ionnickel
Binding capacity/cartridge (GFPuv-6xHis)250 mg