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ROTI®Garose-His/Ni NTA-HPBeads, 100 ml

ROTI®Garose for biochemistry
Examples of effect: Flammable; liquids form compounds with the air which can become explosive; produce flammable gases with water or can self-combust. Safety: Keep away from open flames and sources of heat; close containers tightly; store in a fire-safe manner.
Examples of effect: Lead to damage to heath, cause irritation to eyes, skin or respiratory organs. Are fatal in larger quantities. Safety: As previously stated, in the event of skin irritation or contact with eyes, rinse with water or a suitable medium.
Warning
H226-H319-EUH208
i flammable liquid and vapour, causes serious eye irritation
P210 P280 P303+P361+P353 P305+P351+P338
i keep away from heat. No smoking, wear protective gloves/eye protection, IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water, IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing
Pack Qty.
Pack.
Nickel agarose beads, Agarose beads for affinity chromatography, IMAC matrix/nickel charged, Column matrix for protein isolation, IMAC beads
Density (D) 0,95 g/cm³
Boiling point (bp) >80 °C
Flash point (flp) >50 °C
Storage temp. +4 °C
ADR 3 III
WGK 3
UN-Nr. 1170

For isolation of His-tagged proteins by fast or large scale affinity chromatography under reducing conditions
Suitable for LPLC, FPLC and MPLC and big matrix volumes

Nickel charged NTA-agarose beads for affinity chromatography under reducing conditions. The matrix of choice for applications under medium pressure, with high flow rate or with big sample-/matrix volume. 50 % bead suspension in 20 % ethanol.
In ROTI®Garose-His/Ni NTA-HPBeads, the advantages of the highly efficient Nickel-His binding have been combined with a bead technology allowing a very rapid flow rate.

Immobilized metal ion affinity chromatography (IMAC) still is the most widely used method to obtain high yield of very pure proteins with reasonable effort. ROTI®Garose-His/Ni NTA-HPBeads offer the perfect solution for your specific application under medium pressure (MPLC, FPLC), if rapid flow rates are desired or if big samples volumes have to be purified.
Product details

€1,628.65/Pack Qty. 

excl. VAT. | 100 ml per Pack Qty.

Art. No. 0805.2

Available at short notice
from 6 Pack Qty. €1,547.22/Pack Qty.
from 24 Pack Qty. €1,465.78/Pack Qty.
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Product details



ROTI®Garose-His/Ni NTA-HPBeads ROTI®Garose for biochemistry

Nickel charged NTA-agarose beads for affinity chromatography under reducing conditions. The matrix of choice for applications under medium pressure, with high flow rate or with big sample-/matrix volume. 50 % bead suspension in 20 % ethanol.
In ROTI®Garose-His/Ni NTA-HPBeads, the advantages of the highly efficient Nickel-His binding have been combined with a bead technology allowing a very rapid flow rate.

Immobilized metal ion affinity chromatography (IMAC) still is the most widely used method to obtain high yield of very pure proteins with reasonable effort. ROTI®Garose-His/Ni NTA-HPBeads offer the perfect solution for your specific application under medium pressure (MPLC, FPLC), if rapid flow rates are desired or if big samples volumes have to be purified.

  • Rapid one-step purification of very pure His-tagged proteins from total lysates
  • High binding capacity for 6xHis-tagged protein (≥60 mg/ml purified His-tagged protein)
  • Reliably elution and regeneration
  • Minimized Nickel leaching
  • Compatible with denaturing and reducing reagents
  • For batch mode, gravity flow, MPLC und FPLC
  • Recommended for big matrix volumes

Suitable for

MPLC and FPLC, or if His-tag proteins shall be isolated either in particularly short time or in big amounts from reducing solutions.


Directions for use

The matrix of ROTI®Garose-His/Ni NTA-HPBeads consists of crosslinked and beaded 6 % agarose, NTA-conjugated and charged with divalent Nickel ions. Nickel chelates recognize two exposed histidine tags with good specificity and very high affinity, making the Ni2+-charged matrix the first choice for all standard applications. The tetradentate NTA cross-linker binds his-tagged proteins very efficiently, leading to high recovery rates with minimized nickel bleeding into the eluate. ROTI®Garose-His/Ni NTA-HPBeads may be regenerated, making them very cost-effective.

The Matrix is stable in all commonly used reagents including denaturing reagents (like 8 M urea, 6 M guanidinium hydrochloride) and (dependent on the respective buffer) reducing substances (for instance ≤30 mM Glutathion, ≤10 mM DTT, ≤10 mM DTE, ≤20 mM β-Mercaptoethanol und ≤0,3 % SDS).
May be autoclaved at 121 °C for 30 mins.



Technical Information
Instructions for use, addition Bead suspension, binding capacity ≥60 mg/ml packed matrix (His)6 
ROTI®Garose-His/Ni NTA-HPBeads
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Order No. Pack Qty. Pack. Price Quantity
0805.1 25 ml plastic €510.65
0805.2 100 ml plastic €1,628.65
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Downloads / MSDS


Certificates of Analysis

You can search for and download your certificate of analysis for the selected product here. Please provide your batch number.
The following analysis certificates have been found:

Type analysis

Particle size
Beads (cross-linked agarose)6 %
LigandNTA
Metal ionnickel
Binding capacity (gel) (GFPuv-6xHis)≥60 mg/ml
Ethanol20 %