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ROTI®Garose-Protein A Beads, 25 ml

ROTI®Garose for biochemistry
Examples of effect: Flammable; liquids form compounds with the air which can become explosive; produce flammable gases with water or can self-combust. Safety: Keep away from open flames and sources of heat; close containers tightly; store in a fire-safe manner.
Examples of effect: Lead to damage to heath, cause irritation to eyes, skin or respiratory organs. Are fatal in larger quantities. Safety: As previously stated, in the event of skin irritation or contact with eyes, rinse with water or a suitable medium.
Warning
H226-H319
i flammable liquid and vapour, causes serious eye irritation
P210 P280 P305+P351+P338
i keep away from heat. No smoking, wear protective gloves/eye protection, IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing
Pack Qty.
Pack.
Agarose beads for affinity chromatography, Protein A agarose beads, Column matrix for protein isolation
Density (D) 0,95 g/cm³
Boiling point (bp) >80 °C
Flash point (flp) >50 °C
Storage temp. +4 °C
ADR 3 III
WGK 3
UN-Nr. 1170

For purification of immunoglobulins by affinity chromatography.
For batch mode and gravity flow

Protein A coated agarose beads for low pressure affinity chromatography. High-performance affinity resin for antibody purification. Suspension in 20 % ethanol.

For rapid and simple purification of antibodies with reasonable effort, affinity chromatography still is the first choice in most cases.
Product details

€2,224.20/Pack Qty. 

excl. VAT. | 25 ml per Pack Qty.

Art. No. 1278.2

Available at short notice
from 6 Pack Qty. €2,112.99/Pack Qty.
from 24 Pack Qty. €2,001.78/Pack Qty.
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Product details



ROTI®Garose-Protein A Beads ROTI®Garose for biochemistry

Protein A coated agarose beads for low pressure affinity chromatography. High-performance affinity resin for antibody purification. Suspension in 20 % ethanol.

For rapid and simple purification of antibodies with reasonable effort, affinity chromatography still is the first choice in most cases.

  • One-Step purification of immunoglobulins from cell lysates and biological solutions
  • Binding capacity (human IgG) of approx. 25 mg per ml matrix
  • Superior affinity for IgG from various mammalian species
  • High yield of very pure immunoglobulins
  • Covalent binding of protein A in high ligand density
  • For batch mode and gravity flow column chromatography

Suitable for

Isolation of antibodies, in affinity column chromatography or in batch procedures.


Directions for use

The matrix of ROTI®Garose Protein A Beads consists of cross-linked and beaded 4 % agarose, coated and covalently coupled with protein A from Staphylococcus aureus. The recombinant protein A has high affinity for IgG from a variety of different mammalian species, also binding some populations of IgA and IgM. recProtein A shares IgG binding properties with natural protein A of S. aureus Cowan strain I. Most immunoglobulins may be eluted in 100 mM glycin or citric acid buffer (pH 3,0).



Technical Information
Instructions for use, addition Bead suspension, binding capacity ca. 25 mg/ml Suspension (human IgG) 
ROTI®Garose-Protein A Beads
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Order No. Pack Qty. Pack. Price Quantity
1278.1 5 ml plastic €633.20
1278.2 25 ml plastic €2,224.20
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Downloads / MSDS


Certificates of Analysis

You can search for and download your certificate of analysis for the selected product here. Please provide your batch number.
The following analysis certificates have been found:

Type analysis

Particle size50-150 µm
Beads (cross-linked agarose)4 %
Ligandrecombinant protein A
Chemical bondingcovalent
Ligand density (Protein A)ca. 3 mg/ml
Binding capacity (humane IgG)ca. 25 mg/ml
Stabilizer20 % ethanol

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