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ROTI®Garose-Protein A/G HPBeads, 1 ml

ROTI®Garose for biochemistry
Examples of effect: Flammable; liquids form compounds with the air which can become explosive; produce flammable gases with water or can self-combust. Safety: Keep away from open flames and sources of heat; close containers tightly; store in a fire-safe manner.
Examples of effect: Lead to damage to heath, cause irritation to eyes, skin or respiratory organs. Are fatal in larger quantities. Safety: As previously stated, in the event of skin irritation or contact with eyes, rinse with water or a suitable medium.
Warning
H226-H319
i flammable liquid and vapour, causes serious eye irritation
P210 P280 P305+P351+P338
i keep away from heat. No smoking, wear protective gloves/eye protection, IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing
Pack Qty.
Pack.
Protein A/G agarose beads, Agarose beads for affinity chromatography, Column matrix for protein isolation
Density (D) 0,95 g/cm³
Boiling point (bp) >80 °C
Flash point (flp) >50 °C
Storage temp. +4 °C
ADR 3 III
WGK 3
UN-Nr. 1170

For purification of immunoglobulins by affinity chromatography.
Suitable for LPLC, FPLC and MPLC and big matrix volumes

For rapid and simple purification of antibodies with reasonable effort, affinity chromatography still is the first choice in most cases. ROTI®Garose-Protein A and G Beads offer the perfect solution for your specific application under medium pressure (MPLC, FPLC), if rapid flow rates are desired or if big samples volumes have to be purified.
Product details

€246.20/Pack Qty. 

excl. VAT. | 1 ml per Pack Qty.

Art. No. 0809.2

Available
Ready for dispatch in 3-4 weeks
from 6 Pack Qty. €233.89/Pack Qty.
from 24 Pack Qty. €221.58/Pack Qty.

Product details


For rapid and simple purification of antibodies with reasonable effort, affinity chromatography still is the first choice in most cases. ROTI®Garose-Protein A and G Beads offer the perfect solution for your specific application under medium pressure (MPLC, FPLC), if rapid flow rates are desired or if big samples volumes have to be purified.

  • Rapid one-step purification of immunoglobulins from cell lysates and biological solutions
  • High yield of very pure immunoglobulins
  • Easy elution and regeneration
  • Covalent binding of protein G in high ligand density

The matrix of ROTI®Garose-Protein A and G Beads consists of crosslinked and beaded 4 % agarose, coated and covalently coupled with protein A from Staphylococcus aureus and protein G from Streptococcus, respectively.
The recombinant protein A (recA) shares IgG binding properties with natural protein A of S. aureus Cowan strain I. It has high affinity for IgG from a variety of different mammalian species, also binding some populations of IgA and IgM.
Binding domains specific for albumin as well as cell walls and cell membranes have been removed in the recombinant protein G, in order to ensure the maximum specific IgG binding capacity. It has high affinity for IgG from a variety of different mammalian species, also binding IgG3 with high affinity.


Directions for use

Most immunoglobulins may be eluted in 100 mM glycin or citric acid buffer (pH 3.0). ROTI®Garose-Protein A and G Beads may be regenerated, making them very cost-effective.
May not be autoclaved.



ROTI®Garose-Protein A/G HPBeads ROTI®Garose for biochemistry

Mixture (1:1) of protein A- and protein G coated agarose beads for affinity chromatography under medium pressure, with high flow rate or with big sample-/matrix volume. High-performance affinity resin for antibody purification.
In ROTI®Garose-Protein A/G HPBeads, the advantages of the highly efficient protein A and G/antibody binding have been combined with a bead technology allowing a very rapid flow rate.
50 % bead suspension in 20 % ethanol.

  • Binding capacity (human IgG) of approx. 25 mg per ml matrix
  • Superior affinity for a wide range of IgGs
  • Binding of some populations IgA and IgM
  • Recommended for the isolation of rat IgGs
  • For batch mode, gravity flow, MPLC und FPLC
  • Recommended for big matrix volumes

Suitable for

All small-scale applications, for MPLC and FPLC, or if antibodies shall be isolated either in short time or in big amounts.


Directions for use

The matrix of ROTI®Garose-Protein A/G HPBeads consists of crosslinked and beaded 4 % agarose, coated and covalently coupled with protein A from Staphylococcus aureus and protein G from Streptococcus, respectively.
High binding affinity to IgGs of many different mammalian species, high affinity binding of all IgG3, binding of some populations IgA and IgM.



Technical Information
Instructions for use, addition Bead suspension, binding capacity ca. 25 mg/ml Suspension (human IgG) 
ROTI®Garose-Protein A/G HPBeads
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Art. No. Pack Qty. Pack. Price Quantity
0809.1 0.5 ml plastic

€149.45

0809.2 1 ml plastic

€246.20

0809.3 2 ml plastic

€435.40

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Downloads / MSDS


General information

Further attractive products to complete your chromatography laboratory can be found on our Chromatography page!


Certificates of Analysis

You can search for and download your certificate of analysis for the selected product here. Please provide your batch number.
The following analysis certificates have been found:

Type analysis

Particle size50-150 µm
Beads (cross-linked agarose)4 %
Ligandrecombinant protein A/G
Chemical bondingcovalent
Binding capacity (humane IgG)~25 mg/ml
Ethanol20 %