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ROTI®Garose-Protein G HPBeads, 25 ml

ROTI®Garose for biochemistry
Examples of effect: Flammable; liquids form compounds with the air which can become explosive; produce flammable gases with water or can self-combust. Safety: Keep away from open flames and sources of heat; close containers tightly; store in a fire-safe manner.
Examples of effect: Lead to damage to heath, cause irritation to eyes, skin or respiratory organs. Are fatal in larger quantities. Safety: As previously stated, in the event of skin irritation or contact with eyes, rinse with water or a suitable medium.
Warning
H226-H319
i flammable liquid and vapour, causes serious eye irritation
P210 P280 P305+P351+P338
i keep away from heat. No smoking, wear protective gloves/eye protection, IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing
Pack Qty.
Pack.
Agarose beads for affinity chromatography, Column matrix for protein isolation, Protein G agarose beads
Density (D) 0,95 g/cm³
Boiling point (bp) >80 °C
Flash point (flp) >50 °C
Storage temp. +4 °C
ADR 3 III
WGK 3
UN-Nr. 1170

For purification of immunoglobulins by affinity chromatography.
Suitable for LPLC, FPLC and MPLC and big matrix volumes

Protein G coated agarose beads for affinity chromatography under medium pressure, with high flow rate or with big sample-/matrix volume. High-performance affinity resin for antibody purification. 50% bead suspension in 20 % ethanol.
In ROTI®Garose-Protein G HPBeads, the advantages of the highly efficient protein G/antibody binding have been combined with a bead technology allowing a very rapid flow rate.

For rapid and simple purification of antibodies with reasonable effort, affinity chromatography still is the first choice in most cases. ROTI®Garose-Protein G HPBeads offer the perfect solution for your specific application in small-scale on the bench, under medium pressure (MPLC, FPLC), if rapid flow rates are desired or if big samples volumes have to be purified.
Product details

€1,837.20/Pack Qty. 

excl. VAT. | 25 ml per Pack Qty.

Art. No. 0808.2

Available at short notice
from 6 Pack Qty. €1,745.34/Pack Qty.
from 24 Pack Qty. €1,653.48/Pack Qty.
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Product details



ROTI®Garose-Protein G HPBeads ROTI®Garose for biochemistry

Protein G coated agarose beads for affinity chromatography under medium pressure, with high flow rate or with big sample-/matrix volume. High-performance affinity resin for antibody purification. 50% bead suspension in 20 % ethanol.
In ROTI®Garose-Protein G HPBeads, the advantages of the highly efficient protein G/antibody binding have been combined with a bead technology allowing a very rapid flow rate.

For rapid and simple purification of antibodies with reasonable effort, affinity chromatography still is the first choice in most cases. ROTI®Garose-Protein G HPBeads offer the perfect solution for your specific application in small-scale on the bench, under medium pressure (MPLC, FPLC), if rapid flow rates are desired or if big samples volumes have to be purified.

  • Rapid one-step purification of immunoglobulins from cell lysates and biological solutions
  • High binding capacity for IgG-antibodies of a variety of species
  • High yield of very pure immunoglobulins
  • Easy elution and regeneration
  • Covalent binding of protein G in high ligand density
  • For batch mode, gravity flow, MPLC und FPLC
  • Recommended for big matrix volumes

Suitable for

All small-scale applications, for MPLC and FPLC, or if antibodies shall be isolated either in short time or in big amounts.


Directions for use

The matrix of ROTI®Garose-Protein G HPBeads consists of crosslinked and beaded 4 % agarose, coated and covalently coupled with protein G from Streptococcus. Binding domains specific for albumin as well as cell walls and cell membranes have been removed in the recombinant protein G, in order to ensure the maximum specific IgG binding capacity.
The recombinant protein G has high affinity for IgG from a variety of different mammalian species, unlike protein A also binding IgG3 with high affinity. Protein G is also recommended for use in isolation of rat IgGs. Other immunoglobulin populations, like for instance, IgA, IgE or IgM, are bound with particularly low affinity.
Most immunoglobulins may be eluted in 100 mM glycin or citric acid buffer (pH 3,0).
ROTI®Garose-Protein G HPBeads may be regenerated, making them very cost-effective. May not be autoclaved.



Technical Information
Instructions for use, addition Bead suspension, binding capacity ca. 25 mg/ml Suspension (human IgG) 
ROTI®Garose-Protein G HPBeads
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Order No. Pack Qty. Pack. Price Quantity
0808.1 5 ml plastic €654.70
0808.2 25 ml plastic €1,837.20
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Downloads / MSDS


Certificates of Analysis

You can search for and download your certificate of analysis for the selected product here. Please provide your batch number.
The following analysis certificates have been found:

Type analysis

Particle size
Beads (cross-linked agarose)4 %
Ligandrecombinant protein G
Chemical bondingcovalent
Binding capacity (humane IgG)~20 mg/ml
Ethanol20 %