Agarose Pulsed-Field, 10 g
CAS No. 9012-36-6
€26.50/Pack Qty. Campaign price!
excl. VAT. | 10 g per Pack Qty.
Art. No. 3771.1
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Our agarose ROTI®Garose is a neutral polysaccharide which is obtained in many purification steps from the cell wall of certain algae, called Rhodophyceae. ROTI®Garose forms very clear gels with all standard running buffers and will result in a sharp and clear separation of your biomolecules. Extremely pure agarose with very low interference binding to staining reagents. ROTI®Garose produces a low background and high contrast appearance after staining.
- For clear and sharp bands
- Gels with high transparency
- Low background
- Suitable for all standard running buffers and high-speed buffer systems
- Compatible with all nucleic acid staining systems
- Non-toxic in cell immobilisation assays
- Of course DNase and RNase free
Chemically, agarose is a galactan which is capable of forming extremely solid gels, even at a low concentration. The pore size of the gels is determined by the concentration of the agarose used. Because there are no ionic groups in the gel, hydrophilic materials without any interaction with the gel matrix will also be separated according to their size. The high hysteresis of the agarose, that is the thermal stability after solidification, ensures that the gels remain reliably stable even during heatproducing running conditions.
Agarose Pulsed-Field ROTI®Garose Genetic engineering quality, for pulsed field electrophoresis
- Optimised for separation of fragments of 20 to 50 kb or for Pulsed Field Gel Electrophoresis (PFGE)
- High gel-strength agarose for tearproof gels that are easy to handle
- High DNA mobility for fast and efficient gel electrophoresis
- Low background fluorescence after ethidium bromide staining
- In general, suitable for separation of fragments larger than 1000 bp
- Tested for absence of DNAses, RNAses and DNA binding
Figure (left to right): 0,3 % Agarose Pulsed-Field, extended 1kbp Ladder (1 µg) and High Molecular Weight Marker (0,3 µg, heated for 10 mins. at 65 °C); then same markers on 0,5 % Agarose Pulsed-Field.
|Application||Separation of large fragments (from 20 kb), Pulsed Field gel electrophoresis (PFGE)|
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Osmotic Liquid Migration Caused by Electric Current
In gel matrices of agarose gels, minor impurities caused by cations (sulphate ester) are frequent. When an electric field is applied they move in the direction of the cathode, generating an electroosmotic flow (EOF) of the entire aqueous medium towards the negative pole. The migration speed of the flow depends, amongst other things, on the strength of the electric field and the frictional force generated by the gel matrix. The EOF always runs in the direction of the cathode, that is opposite to the movement of the anions separated during nucleic acid electrophoresis, and slows down their migration.
In the event of an agarose with high EEO, many cations are present, which generate a strong EOF. Furthermore, the gel strength is low, causing only weak frictional force. The strong EOF can lead to a change of behaviour during the run of the negatively-charged molecules (DNA/RNA) when using the agarose MEEO or HEEO. In extreme case anions with low self-mobility can even be diverted and transported in the direction of the cathode, causing band shifting.
Roth Has the Right Agarose for Every Application:
|Standard||3810||Routine gels, student’s courses, general analyses (1-20 kb)|
|NEEO ultra quality||2267||All standard applications, qualitative and quantitative gels, screening and blotting. Range 500 bp-20 kb|
|Agarose Tablets||HP67||Highly reproducible gels, or simple applications in students courses. Suitable for all standard-gels (0,5-0,25 %). For fragments ≥300 bp|
|GTQ||6352||Genetic engineering quality, for DNA-elution of fragments ≥500 bp without melting the agarose** Tip: Use Kit Roti®-Prep Gel Extraction (Art. No. 8510.1)|
|Broad Range||T846||For the total analytical range (200 bp up to 40 kb), Pulsed-Field electrophoresis (PFGE), blotting, shift assays. Ideal when only a few agaroses are to be used in the laboratory.|
|Pulsed-Field||3771||Separation of large fragments (from 20 kb), Pulsed Field gel electrophoresis (PFGE)|
|HR PLUS||HP30||Analysis of fragments between 100 and 3000 bp|
|High Resolution||K297||Analysis of fragments between 50 and 1000 bp|
|Low Melt||6351||With low melting and gelling temperatures (MT ≤65,5 °C, GT ≤28 °C). For gel elution from melted agarose. Range 500 bp-20 kb.|
|LM / PCR||HP31||Genetic engineering quality with low melting and gelling temperatures (MT ≤65 °C, GT ≤35 °C). For DNA-elution of fragments <1500 bp from melted agarose.|
|Super LM||HP45||With extremely low melting and gelling temperatures. (MT ≤62 °C, GT ≤20 °C). For gel elution from melted agarose. Recommended for in-gel analysis, capillary electrophoresis and cell and tissue culture. For fragments ≥1000 bp.|
|MEEO Ultra quality||2268||With medium EEO. For immune, serum and antibody electrophoresis. Range 500 bp-10 kb.|
|HEEO Ultra quality||2269||With high EEO. For protein precipitation and countercurrent electrophoresis.|
|Synergel®||0184||Agarose additive for finer pore formation. Increases the separative power of the agarose. For fragments from 10 bp.|
|Sulphate (SO4)||≤0,10 %|
|Loss on drying||≤10 %|
|Gel strength (1 % gel)||≥1800 g/cm2|
|Gelling temperature||36 ±1,5 °C|
|Melting temperature||≥90 °C|
|DNases, RNases||none detected|