Technical Data Sheet
Synergel™, 100 g
Transport temp. ambient temp.
CAS No. 9000-40-2
excl. VAT. | 100 g per Pack Qty.
Art. No. 0184.1
Fits to / Accessories
- Enhances separation of nucleic acid fragments
- For extremely clear gels
- Facilitates image documentation
- Optimal display especially with small fragments
1. What percentage agarose gel is currently being used?
2. The gel will inevitably contain 0.7 % agarose (the minimum agarose concentration required to ensure gel stability). Hence, subtract 0.7 % from the agarose total.
3. Divide the difference by 2. The result is the percentage of Synergel™ to be added to the 0.7 % agarose.
Convert a 1 % agarose to a 1 % Synergel™/agarose gel
a. Substract 0.7 % from the 1 % agarose: 1 % - 0.7 % = 0.3 %
b. Divide the difference by 2: 0.3 % : 2 = 0.15 %
c. 0.15 % (150 mg / 100 ml) is the amount of Synergel™ added to 0.7 % agarose (0.7 g / 100 ml) to produce the functional equivalent of a 1 % agarose gel.
Synergel™ ≥99 %, for gel electrophoresis, up to 30 kb
Synergel™ is a synergistic gelling and sieving agent consisting of a modified polysaccharide which, when combined with agarose, forms a hydrogen bonded binary gel system.
The addition of Synergel™ to agarose improves gel performance by providing superior separation and definition of DNA fragments up to 30 kb. A gel mixture containing 0,7 % agarose and 0,7 % Synergel™, e.g., will produce improved results when compared to a 2 % agarose-only gel.
Synergel™ also provides greater optical clarity, which allows higher quality photodocumentation of stained gels.
Standard buffer systems like phosphate, acetate or borate buffered Tris-EDTA may be used in forming the gel with agarose/ Synergel™. RNA may also be separated in this system by using a 2,2 M formaldehyde-containing buffer. Synergel™ is compatible with standard blotting procedures, thus, nucleic acid may be electroeluted or transferred to membranes following standard Southern/Northern blotting protocols.
The figure compares separation qualities of
A: 1,5 % Synergel™ / 0,7 % agarose and B: 4 % high-resolution agarose (NuSieve®).
Please see also figures for pUC19 markers T149 and X901.
|Application||Agarose additive for finer pore formation. Increases the separative power of the agarose. For fragments from 10 bp|
- Subtotal: 0.00
|Art. No.||Pack Qty.||Pack.||Price||Quantity|
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Osmotic Liquid Migration Caused by Electric Current
In gel matrices of agarose gels, minor impurities caused by cations (sulphate ester) are frequent. When an electric field is applied they move in the direction of the cathode, generating an electroosmotic flow (EOF) of the entire aqueous medium towards the negative pole. The migration speed of the flow depends, amongst other things, on the strength of the electric field and the frictional force generated by the gel matrix. The EOF always runs in the direction of the cathode, that is opposite to the movement of the anions separated during nucleic acid electrophoresis, and slows down their migration.
In the event of an agarose with high EEO, many cations are present, which generate a strong EOF. Furthermore, the gel strength is low, causing only weak frictional force. The strong EOF can lead to a change of behaviour during the run of the negatively-charged molecules (DNA/RNA) when using the agarose MEEO or HEEO. In extreme case anions with low self-mobility can even be diverted and transported in the direction of the cathode, causing band shifting.
Roth Has the Right Agarose for Every Application:
|Standard||3810||Routine gels, student’s courses, general analyses (1-20 kb)|
|NEEO ultra quality||2267||All standard applications, qualitative and quantitative gels, screening and blotting. Range 500 bp-20 kb|
|Agarose Tablets||HP67||Highly reproducible gels, or simple applications in students courses. Suitable for all standard-gels (0,5-0,25 %). For fragments ≥300 bp|
|GTQ||6352||Genetic engineering quality, for DNA-elution of fragments ≥500 bp without melting the agarose** Tip: Use Kit Roti®-Prep Gel Extraction (Art. No. 8510.1)|
|Broad Range||T846||For the total analytical range (200 bp up to 40 kb), Pulsed-Field electrophoresis (PFGE), blotting, shift assays. Ideal when only a few agaroses are to be used in the laboratory.|
|Pulsed-Field||3771||Separation of large fragments (from 20 kb), Pulsed Field gel electrophoresis (PFGE)|
|HR PLUS||HP30||Analysis of fragments between 100 and 3000 bp|
|High Resolution||K297||Analysis of fragments between 50 and 1000 bp|
|Low Melt||6351||With low melting and gelling temperatures (MT ≤65,5 °C, GT ≤28 °C). For gel elution from melted agarose. Range 500 bp-20 kb.|
|LM / PCR||HP31||Genetic engineering quality with low melting and gelling temperatures (MT ≤65 °C, GT ≤35 °C). For DNA-elution of fragments <1500 bp from melted agarose.|
|Super LM||HP45||With extremely low melting and gelling temperatures. (MT ≤62 °C, GT ≤20 °C). For gel elution from melted agarose. Recommended for in-gel analysis, capillary electrophoresis and cell and tissue culture. For fragments ≥1000 bp.|
|MEEO Ultra quality||2268||With medium EEO. For immune, serum and antibody electrophoresis. Range 500 bp-10 kb.|
|HEEO Ultra quality||2269||With high EEO. For protein precipitation and countercurrent electrophoresis.|
|Synergel®||0184||Agarose additive for finer pore formation. Increases the separative power of the agarose. For fragments from 10 bp.|
|Colour||white to off-white powder|
|pH (1 % solution)||9|
|DNase, RNase||none detected|