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Bovine Serum Albumin (BSA) Fraction V, fatty acid-free, US-Origin, 100 g

≥98 %, fatty acid-free, for biochemistry and molecular biology
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Fraction V, BSA Bovine Serum Albumin
Molar mass (M) ~66 000 g/mol
Storage temp. +4 °C
WGK 1
CAS No. 9048-46-8
EG-Nr. 232-936-2

Suitable as blocking or stabilising reagent in all fatty acid-sensitive assays.
BSA "Fraction V".
Recommended if BSA with US-Origin is required.

For purification of this Albumin an extensive heat-shock/diafiltration method is used, which has been shown to bring more highly purified products than the standard process acc. to Cohn. The process is taking place in a closed system.
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€532.15/Pack Qty. 

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Art. No. 9638.4

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For purification of this Albumin an extensive heat-shock/diafiltration method is used, which has been shown to bring more highly purified products than the standard process acc. to Cohn. The process is taking place in a closed system.


Molar extinction coefficient: 4,4x104 L x mol-1 x cm-1 (acc. to 0,667 L x mol-1 x cm-1 for a 0,1 % solution).



Bovine Serum Albumin (BSA) Fraction V, fatty acid-free, US-Origin ≥98 %, fatty acid-free, for biochemistry and molecular biology

Highly pure albumin, prepared from blood of cattle certified for US-origin.
Albumin, fatty acid-free, US-Origin, may well be used for blocking of all assays regarding membrane proteins, or other approaches aiming for fatty acid-associated proteins. It is also well suited for stabilisation of antibodies, enzymes or fatty acids. Albumin, fatty acid-free, US-Origin, shows exceedingly low content of endotoxins and IgGs, making it a useful reagent for cell biology also.


Tested for very low fatty acid concentration.



Bovine Serum Albumin (BSA) Fraction V, fatty acid-free, US-Origin
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Downloads / MSDS


General information

Recommended Application of Bovine Serum Albumins (BSA)

BSA Art. No. Recommended Application
Albumin Solution 20 %, Europe 9400 Stabilization of proteins in general, especially antibodies. Suitable for all biochemical assays, e. g. as protein standard or blocking reagent, unless special requirements apply.
Albumin Solution 30 %, Europe 9401 Stabilization of proteins in general, especially antibodies. Suitable for all biochemical assays, e. g. as protein standard or blocking reagent, unless special requirements apply.
Albumin Solution 30 %, with azide, US-Origin 9414 Stabilization of proteins in general, especially antibodies. Suitable for all biochemical assays, e. g. as protein standard or blocking reagent, unless special requirements apply. Recommended if albumin with certified origin USA has to be used.
Fraction V, Europe 1ETA Stabilisation of proteins and blocking reagent in protein biochemical detection systems. Basically suitable for all assays unless specific requirements apply.
Fraction V, US-Origin 3854 Stabilisation of proteins and blocking reagent in all standard biochemical detection systems. Basically suitable for all assays unless specific requirements apply. Recommended if albumin with certified origin USA has to be used.
Fraction V, NZ-Origin 8076 Stabilisation of proteins and blocking reagent in protein biochemical detection systems. Basically suitable for all assays unless specific requirements apply. Recommended if albumin with certified origin New Zealand or Australia has to be used.
Fraction V, pH 5.2, Europe 2834 Stabilisation of proteins and blocking reagent. Particularly recommended for Western-Blotting and ELISAs.
Fraction V, pH 5.2, US-Origin 1ET8 Stabilisation of proteins and blocking reagent. Particularly recommended for Western-Blotting and ELISAs. Recommended if albumin with certified origin USA has to be used.
Fraction V, sufhydryl-blocked, NZ-Origin 1ET3 Particularly sensitive assays where monomeric albumin is guaranteed to be used or where reproducibility is particularly important. Can be stored as monomers over a longer period of time.
Fraction V, biotin-free, NZ-Origin 0163 Stabilisation of proteins and blocking reagent in all biotin / streptavidin-mediated detection systems (membrane-bound- or coating-plate-assays, as well as soluble assays).
Fraction V, protease-free, Europe T844 Stabilisation of proteins in general, antibodies in particular, and blocking reagent in sensitive enzymatic detection systems, activity tests or protein/protein-interaction assays.
Fraction V, protease-free, US-Origin 1ET5 Stabilisation of proteins in general, antibodies in particular, and blocking reagent in sensitive enzymatic detection systems, activity tests or protein/protein-interaction assays. Recommended if albumin with certified origin USA has to be used.
Fraction V, protease-free, NZ-Origin 1ET7 Stabilisation of proteins in general, antibodies in particular, and blocking reagent in sensitive enzymatic detection systems, activity tests or protein/protein-interaction assays. Recommended if albumin with certified origin New Zealand or Australia has to be used.
Fraction V, IgG-free, US-Origin 1ET9 Stabilisation of antibodies. Blocking reagent in all antibody-mediated detection systems and Radio-Immuno Assays (RIAs). Reduces false-positive signals. Recommended if albumin with certified origin USA has to be used.
Fraction V, IgG-free, NZ-Origin 3737 Stabilisation of antibodies. Blocking reagent in all antibody-mediated detection systems and Radio-Immuno Assays (RIAs). Reduces false-positive signals. Recommended if albumin with certified origin New Zealand or Australia has to be used.
Fraction V, fatty acid-free, Europe 0052 Stabilisation of proteins and blocking reagent in detection systems. Particularly recommended for experiments on metabolism proteins, membrane-associated proteins and cell surface proteins.
Fraction V, fatty acid-free, US-Origin 9638 Particularly for experiments on metabolism proteins, membrane-associated proteins and cell surface proteins. Also well suited for cell biology and all antibody-mediated detection systems. Recommended if albumin with certified origin USA has to be used.
Fraction V, fatty acid-free, NZ-Origin 1ETC Stabilisation of proteins and blocking reagent in detection systems. Particularly for experiments on metabolism proteins, membrane-associated proteins and cell surface proteins. Recommended if albumin with certified origin New Zealand or Australia has to be used.
Fraction V, very low endotoxin, US-Origin 1ET6 Cell culture of all cell lines, primary cells, stem cells. Cloning and 3D-cultures or cell-stressing assays like transfections, in vitro infections, cell fusion. Recommended if albumin with certified origin USA has to be used.
Fraction V, very low endotoxin, NZ-Origin 1ET4 Cell culture of all cell lines, primary cells, stem cells. Cloning and 3D-cultures or cell-stressing assays like transfections, in vitro infections, cell fusion. Recommended if albumin with certified origin New Zealand or Australia has to be used..

Molar extinction coefficient: 4,4x104 L x mol-1 x cm-1 (acc. to 0,667 L x mol-1 x cm-1 for a 0,1 % solution).


BSA is a soluble monomeric protein with a molecular weight of 66.5 kDa. It is synthesized in the liver and extracted from the blood of cattle. In the bloodstream it serves as a binding and transport protein. BSA is used as a standard in protein quantification, as a blocking reagent, as a satabilizer in molecular applications and as a nutrient additive in cell cultures.


Purification process

The purification of the BSA is carried out using a far-reaching heat shock/diafiltration method that produces higher purity albumins than the usual Cohn crystallization. The process takes place in a closed system.


Albumin, NZ-Origin is particularly recommended when procedural regulations require the use of albumin of guaranteed origin Australia or New Zealand.


BSA, US-Origin is particularly recommended if the use of albumin with certified US-Origin is dictated by process regulations.


Albumin of European origin is produced from blood of European cattle herds. It is excellent for use in all assays and products manufactured for regions outside the USA, Australia and New Zealand.


Certificates of Analysis

You can search for and download your certificate of analysis for the selected product here. Please provide your batch number.
The following analysis certificates have been found:

Guarantee analysis

Appearanceflakes
Colourpale yellow to beige
OriginUSA
Assay≥98 %
Protein≥96 %
Water content≤3 %
Heavy metals≤2 ppm
pH value (10 % in H2O)6.0-7.5
IgG (COBAS)none detected
Endotoxin content≤5 E.U./mg
Total fatty acids≤0.01 %