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ROTI®Lyte pH 3–7, 10 ml

40 % in water, sterile filtered (0,2 μm)
Icon_Steril Sterile products
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Pack Qty.
Ampholyte, Carrier Ampholyte
Density (D) 1,12 g/cm³
Storage temp. +4 °C

Carrier ampholytes for isoelectric focussing (IEF).
Added in 3–5% working concentration to acrylamide- or agarose gel solution.

Type analysis

€209.65/Pack Qty. 

excl. VAT. | 10 ml per Pack Qty.

Art. No. 5839.1

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Product details

  • Reliable formation of a finely graded linear pH gradient
  • Sharp band resolution due to high buffer capacity at pI
  • High conductivity, no overheating of the gel
  • Short staining- and destaining periods
  • Compatible with all standard staining methods

ROTI®Lyte carrier ampholytes consist of a diverse mixture of sterile filtered hydrous polyamino-polysulfone-polycarboxylic acids. Due to the multitude of isoelectric points (pIs) present, those amphoteric molecules form a very finely graded, linear pH gradient in the electric field, hence making them particularly suitable for isoelectric focusing (IEF).

ROTI®Lyte carrier ampholytes excel by their high buffer capacity and conductivity, leading to excellent band definition. Even proteins differing by just 0.01 pH units may be discriminated efficiently. Due to their low molecular weight of 1000 Da in maximum, the ampholytes disperse quickly in the gel, therefore guaranteeing an even distribution of sample proteins during IEF. Since there is virtually no unspecific binding of dyes, no background staining happens, and ROTI®Lyte ampholytes are perfectly compatible with all standard staining methods. They are well soluble in trichloroacetic acid, and may easily be removed from the gel during subsequent fixation.

Directions for use

Store unopened bottles at +4 °C. Opened bottles should be aliquoted and stored at –20 °C. Avoid frequent freeze and thaw cycles.

ROTI®Lyte pH 3–7 40 % in water, sterile filtered (0,2 μm)

Figure (example 5841.1):
pH range 3–10, gel thickness 350 µm
Lanes 1, 2, 5, 6: Potato extracts (10 µl)
Lanes 3, 4: Protein standard (10 µl)

Technical Information
Conductivity 0,2–0,7  mS/cm
pH upper limit 7,0 ±0,5  
pH lower limit 3,3 ±0,3  
ROTI®Lyte pH 3–7
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Order No. Pack Qty. Pack. Price Quantity
5839.1 10 ml glass €209.65
5839.2 25 ml glass €461.20
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Analysis certificates

You can search for and download your certificate of analysis for the selected product here. Please provide your batch number.
The following analysis certificates have been found:

Type analysis

Appearanceclear solution  
Content (w/v)40 %  
pH-value (2,5 % in H2O, 20 °C)4,5-5,5  
Conductivity (2,0 % in H2O, 20 °C)0,20-0,70 mS/cm  
pH Range3,3 ±0,3 to 7,0 ±0,5  
pH gradient performedmin. 70 %