Technical Data Sheet
ROTI®Cell Antibiotic-Antimycotic
The products marked accordingly are shipped frozen in special, sustainable cooling boxes with -21 °C PCM cooling packs.
Sterile products
Ready-to-use products and reagents
Boiling point (bp) 100 °C
Storage temp. -20 °C
Transport temp. frozen
WGK 1
Thaw overnight at +2 to +8 °C and homogenize by gently swirling.
For cell culture
€42.90/Pack Qty.
excl. VAT. | 100 ml per Pack Qty.
Art. No. 3A8X.1
Product details
The Antibiotic-Antimycotic Solution is a ready-to-use solution used in cell culture to prevent both bacterial and fungal contamination. In addition to penicillin and streptomycin, this solution also contains amphotericin B.
Penicillin, originally derived from the Penicillium fungus, inhibits bacterial cell wall synthesis by blocking the activity of the enzyme D-alanine transpeptidase, which is responsible for the synthesis of cell wall components. This effect mainly occurs in cells that are actively dividing.
Streptomycin, isolated from Streptomyces griseus, works by binding to the 30S subunit of the bacterial ribosome, thus inhibiting protein synthesis. This mechanism leads to bacterial cell death, regardless of whether the cells are dividing or not.
Amphotericin B is a fungicide that prevents the growth of fungi and yeasts by increasing the permeability of the fungal membrane. Pores are formed, allowing essential ions to leak out of the cell, leading to cell death.
ROTI®Cell Antibiotic-Antimycotic CELLPURE® 100x, sterile
A solution of antibiotics and antimycotics to prevent contamination by bacteria and fungi in cell culture.
Penicillin: 10.000 Units/ml
Streptomycin: 10.000 µg/ml
Amphotericin B: 25 µg/ml
| Instructions for use, addition | Preparation of aliquots recommended to avoid repeated freeze-thaw cycles. |
- Subtotal: 0.00
| Art. No. | Pack Qty. | Pack. | Price | Quantity | |
|---|---|---|---|---|---|
| 3A8X.1 | 100 ml | plastic |
€42.90 |
|
|
|
In stock
Available (note delivery time)
In procurement
No longer available
Delivery date currently unknown
|
|||||
- Subtotal: 0.00
Downloads / MSDS
General information
In addition to the various nutrients in the medium, the pH value also plays a very important role in cell culture. The physiological pH for almost all mammalian cells is 7.4. Deviating from this, it has been observed that certain transformed cell lines prefer a pH between 7.0 and 7.4 and certain fibroblasts prefer a pH between 7.4 and 7.7. In any case, pH fluctuations should be avoided and the pH should be maintained as well as possible. That is why a buffer system in media is necessary. The most important buffer system in the body is the carbonic acid-carbonate buffer system. Accordingly, almost all cell culture media contain bicarbonate (NaHCO3). Bicarbonate alone leads to a strong pH increase of the medium, so that NaHCO3-buffered media must always be used with CO2 incubators. Depending on the NaHCO3 concentration in the medium, different CO2 environments are necessary to ensure a pH of about 7.4. For this reason, you should not work outside of the CO2 incubator for too long. If this is necessary, a HEPES buffer should be used in addition to the NaHCO3, which contributes as a second buffer system to stabilize the pH value.
Certificates of Analysis
Type analysis
| pH value | 6.0-7.5 |
| Osmolality | 300-400 mOsm/kg |
| Endotoxins | ≤10 EU/ml |
| Sterility | complies |