ROTI®Block, 250 ml
Boiling point (bp) 100 °C
Storage temp. +15 to +25 °C
Transport temp. ambient temp.
Contains Tween® 20
excl. VAT. | 250 ml per Pack Qty.
Art. No. A151.1
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- Western- and Far Western Analysis
- Southern- and Northern-Blots
- ELISA- and Sandwich-Assays
- Precoating of ELISA-plates (incl. drying)
- in situ hybridisation
- Microarray hybridisation
In order to obtain high signal intensity, nonspecific antibody binding sites must be blocked. Whilst detergents are not effective as blocking agents, the addition of proteins such as BSA may mask specific binding sites.
ROTI®Block is an innovative, polymer-based blocking reagent which solves such problems. With ROTI®Block, the specific binding sites remain accessible while nonspecific reactions are suppressed, thus leading to an increase in signal intensity.
ROTI®Block 10x conc., ready-to-use
Included you will find a detailed instruction manual with protocols, e.g. for stripping ROTI®PVDF-membranes from bound probes without destabilising the blocking, which enables fast rescreening of the filter.
ROTI®Block also enables the blot to be Ponceau stained even after blocking.
For detection systems using antibodies specifically directed against protein-phosphorylations we recommend to test and optimise the blocking efficiency prior to assaying your samples.
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