Jump to content Jump to navigation menu

Transfer membrane Amersham™ Protran® NC 0.45 disks Ø8.2 cm

From  Cytiva
Examples of effect: Flammable; liquids form compounds with the air which can become explosive; produce flammable gases with water or can self-combust. Safety: Keep away from open flames and sources of heat; close containers tightly; store in a fire-safe manner.
Warning
H228
i flammable solid
P210
i keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking
Material: nitrocellulose (100 %).
Related products
Dimensions
Flash point (flp) 13 °C
WGK 1


€233.60/Pack Qty. 

excl. VAT. | 50 sheet per Pack Qty.

Art. No. 4592.1

Not available
from 6 Pack Qty. €221.92/Pack Qty.
from 24 Pack Qty. €210.24/Pack Qty.
Carl Roth subscription service

Now recurring orders conveniently delivered as a subscription!

With the new Carl Roth Replenishment Service you can let products be ordered automatically which you need regularly in your lab!

How it works:
1

Put all products for your subscription in the desired quantity in the basket.

2

In the shopping cart, select the option "Order shopping cart as subscription" Order as subscription.

3

Set Starty point and interval for your subscription and submit order!

By the way: Through your account you can customize or delete your subscriptions anytime.

Product details

Material: nitrocellulose (100 %).


Transfer membrane Amersham™ Protran® NC 0.45 disks Ø8.2 cm 

Technical Information
Dimensions Ø8.2 cm 
Version disks 
Pore size 0,45 µm
Type Protran® NC 0,45 
Bond electrostatic, hydrophobic 
Binding capacity 86-110 µg/cm²
Immobilisation UV, baking (max. 80 °C) 
Transfer membrane Amersham™ Protran® NC 0.45 disks Ø8.2 cm
Selected quantity:   0
  1. Subtotal:  0.00
Order No. Dimensions Pack Qty. Price Quantity
4592.1 Ø8.2 cm 50 sheet

€233.60

In stock
In procurement
Not available
Delivery date currently unknown
Selected quantity:   0
  1. Subtotal:  0.00

Downloads / MSDS

General information

Technical Parameters of ROTH-Transfer Membranes

Membrane Material Characteristics Thickness (μm) Binding capacity (μg/cm2) Pore size (μm) Immobilisation
Roti® Fluoro PVDF Polyvinyliden fluoride hydrophobic 125 ±25 300 0,2 not necessary
Roti® PVDF 0.2 Polyvinyliden fluoride hydrophobic 140 ±20 ≥125 0,2 not necessary
Roti® PVDF Polyvinyliden fluoride hydrophobic 125 ±25 ca. 200 0,45 not necessary
Immobilon P Polyvinyliden fluoride hydrophobic 120 ±25 150-300 0,45 not necessary
Roti® Nylon 0.2 Nylon 6,6 neutral/amphoteric 150 ±20 500 0,2/0,45 UV, baking
Roti® Nylon plus Nylon 6,6 hydrophilic, positively charged 160 ±20 500 0,45 not necessary, UV and baking possible
Roti® NC Nitrocellulose electrostatic, hydrophobic 150 ±50 125-200 0,2 UV, baking (max. 80 °C)
Roti® NC 0.45 Nitrocellulose electrostatic, hydrophobic 150 ±10 ≥125 0,45 UV, baking (max. 80 °C)
Amersham Protran® Nitrocellulose electrostatic, hydrophobic unknown 11-72 0,2/0,45 UV, baking (max. 80 °C)
Amersham Protran® supported Nitrocellulose electrostatic, hydrophobic unknown 86-110 0,2/0,45 UV, baking (max. 80 °C)

Size Comparison of Membrane Pores and Biomolecules

Biomolecules / Membrane Pores Size (kD)
genomic DNA (human) >11000000
E. coli approx. 1500000
0.45 μm approx. 5000
0.2 μm approx. 2000
DNA 3 kb approx. 1000
RNA 1 kb approx. 300
Antibodies (IgG) approx. 150
Proteins in the lab approx. 10–300
BSA (Albumin) 69

Application of ROTH-Transfer Membranes

Biomolecules Nylon plus Nylon 0.2 PVDF 0.2 PVDF Fluoro PVDF NC NC 0.45
DNA +++ + - - - + +
RNA + +++ - - - + +
Proteins - + +++ +++ +++ + +
Detection System
Colour +++ +++ +++ +++ +++ +++ +++
Radioactivity +++ +++ +++ +++ +++ +++ +++
Chemoluminescence +++ +++ +++ +++ - + +
Fluorescence +++ +++ - - +++ - -
+++: recommended +: suitable -: not

Certificates of Analysis

You can search for and download your certificate of analysis for the selected product here. Please provide your batch number.
The following analysis certificates have been found: