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Transfer membrane Amersham™ Protran® NC 0.45 roll 400 × 30 cm

From  Cytiva
Examples of effect: Flammable; liquids form compounds with the air which can become explosive; produce flammable gases with water or can self-combust. Safety: Keep away from open flames and sources of heat; close containers tightly; store in a fire-safe manner.
Warning
H228
i flammable solid
P210
i keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking
Material: nitrocellulose (100 %).
Related products
Dimensions
Flash point (flp) 13 °C
WGK 1


Transfer membrane for most nucleic acid and protein immobilising techniques

Replaces Protran® BA 85 from Whatman®

Multiple use - ideal for most nucleic acid and protein immobilising techniques. Pure nitro-cellulose, free from cellulose acetate and triton. They have a high binding capacity and low background.
Product details

€431.10/Pack Qty. 

excl. VAT. | 1 roll(s) per Pack Qty.

Art. No. 4675.1

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from 6 Pack Qty. €409.54/Pack Qty.
from 24 Pack Qty. €387.99/Pack Qty.
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Product details

Material: nitrocellulose (100 %).

Transfer membrane for most nucleic acid and protein immobilising techniques

Replaces Protran® BA 85 from Whatman®

Multiple use - ideal for most nucleic acid and protein immobilising techniques. Pure nitro-cellulose, free from cellulose acetate and triton. They have a high binding capacity and low background.


Directions for use

Nitrocellulose should only conditionally be used for electrophoretic transfer of nucleic acids, because of the high salt concentrations required for the transfer.
Membranes of 0,45 μm pore size allow for DNA transfer (minimum 300 bp) and protein transfer (minimum 20 kDa).


Application examples

Southern, Northern, Western transfer, colony and plaque hybridisation, immunoblots, nucleic acid and protein dot-blot, slot-blot, electrophoretic transfer of proteins.
Detection systems are radioactivity, chemoluminescence, colour



Transfer membrane Amersham™ Protran® NC 0.45 roll 400 × 30 cm 

In practical roll format for maximum flexibility in cutting.



Technical Information
Dimensions 400 × 30 cm 
Version roll 
Pore size 0,45 µm
Type Protran® NC 0,45 
Bond electrostatic, hydrophobic 
Binding capacity 86-110 µg/cm²
Immobilisation UV, baking (max. 80 °C) 
Transfer membrane Amersham™ Protran® NC 0.45 roll 400 × 30 cm
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Order No. Dimensions Pack Qty. Price Quantity
4675.1 400 × 30 cm 1 roll(s)

€431.10

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Downloads / MSDS

General information

Technical Parameters of ROTH-Transfer Membranes

Membrane Material Characteristics Thickness (μm) Binding capacity (μg/cm2) Pore size (μm) Immobilisation
Roti® Fluoro PVDF Polyvinyliden fluoride hydrophobic 125 ±25 300 0,2 not necessary
Roti® PVDF 0.2 Polyvinyliden fluoride hydrophobic 140 ±20 ≥125 0,2 not necessary
Roti® PVDF Polyvinyliden fluoride hydrophobic 125 ±25 ca. 200 0,45 not necessary
Immobilon P Polyvinyliden fluoride hydrophobic 120 ±25 150-300 0,45 not necessary
Roti® Nylon 0.2 Nylon 6,6 neutral/amphoteric 150 ±20 500 0,2/0,45 UV, baking
Roti® Nylon plus Nylon 6,6 hydrophilic, positively charged 160 ±20 500 0,45 not necessary, UV and baking possible
Roti® NC Nitrocellulose electrostatic, hydrophobic 150 ±50 125-200 0,2 UV, baking (max. 80 °C)
Roti® NC 0.45 Nitrocellulose electrostatic, hydrophobic 150 ±10 ≥125 0,45 UV, baking (max. 80 °C)
Amersham Protran® Nitrocellulose electrostatic, hydrophobic unknown 11-72 0,2/0,45 UV, baking (max. 80 °C)
Amersham Protran® supported Nitrocellulose electrostatic, hydrophobic unknown 86-110 0,2/0,45 UV, baking (max. 80 °C)

Size Comparison of Membrane Pores and Biomolecules

Biomolecules / Membrane Pores Size (kD)
genomic DNA (human) >11000000
E. coli approx. 1500000
0.45 μm approx. 5000
0.2 μm approx. 2000
DNA 3 kb approx. 1000
RNA 1 kb approx. 300
Antibodies (IgG) approx. 150
Proteins in the lab approx. 10–300
BSA (Albumin) 69

Application of ROTH-Transfer Membranes

Biomolecules Nylon plus Nylon 0.2 PVDF 0.2 PVDF Fluoro PVDF NC NC 0.45
DNA +++ + - - - + +
RNA + +++ - - - + +
Proteins - + +++ +++ +++ + +
Detection System
Colour +++ +++ +++ +++ +++ +++ +++
Radioactivity +++ +++ +++ +++ +++ +++ +++
Chemoluminescence +++ +++ +++ +++ - + +
Fluorescence +++ +++ - - +++ - -
+++: recommended +: suitable -: not

Certificates of Analysis

You can search for and download your certificate of analysis for the selected product here. Please provide your batch number.
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