Technical Data Sheet
Transfer membrane ROTI®Nylon plus, 20 × 20 cm
€235.45/Pack Qty.
excl. VAT. | 10 unit(s) per Pack Qty.
Art. No. HP36.1
Fits to / Accessories
Product details
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Transfer membrane ROTI®Nylon plus
Ideal for DNA/RNA-transfers.
ROTI®Nylon plus is a cationic, einforced nylon 6.6-membrane with extremely high tensile strength. The positively charged membrane surface guarantees rapid and efficient binding of DNA and RNA. May be steam sterilised.
Also available in convenient pre-cut sheets of standard gel size!
Nucleic acids can be cross-linked to the membrane by baking (80 °C, 15 min) or through UV exposure. No cross-linking is required for alkaline transfer as the negatively charged DNA forms a very stable complex with the positively charged ROTI®Nylon membrane.
Detection systems: radioactivity, chemoluminescence, fluorescence
- Southern and Northern blot
- Dot-Blot
- DNA fngerprinting
- Macro- and Microarrays
| Dimensions | 20 × 20 cm |
| Version | sheet |
| Pore size | 0.45 µm |
| Type | 0.45 µm |
| Bond | hydrophilique |
| Load | positive |
| Binding capacity | 500 µg/cm² |
| Thickn. | 160±20 µm |
| Immobilisation | not necessary, UV and baking possible |
- Subtotal: 0.00
| Art. No. | Dimensions | Pack Qty. | Price | Quantity | |
|---|---|---|---|---|---|
| HP35.1 | 15 × 10 cm | 10 unit(s) |
€161.70 |
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| HP36.1 | 20 × 20 cm | 10 unit(s) |
€235.45 |
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| K058.1 | 300 × 30 cm | 1 roll(s) |
€339.00 |
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In stock
Available
In procurement
No longer available
Delivery date currently unknown
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- Subtotal: 0.00
Downloads / MSDS
General information
Technical Parameters of ROTH-Transfer Membranes
| Membrane | Material | Characteristics | Thickness (μm) | Binding capacity (μg/cm2) | Pore size (μm) | Immobilisation |
| Roti® Fluoro PVDF | Polyvinyliden fluoride | hydrophobic | 125 ±25 | 300 | 0,2 | not necessary |
| Transfer membrane ROTI®PVDF 0.2 | Polyvinyliden fluoride | hydrophobic | 190 ±50 | ≥125 | 0,2 | not necessary |
| Transfer membrane ROTI®PVDF 0.45 | Polyvinyliden fluoride | hydrophobic | 190 ±50 | ≥125 | 0,45 | not necessary |
| Immobilon P | Polyvinyliden fluoride | hydrophobic | 120 ±25 | 150-300 | 0,45 | not necessary |
| Roti® Nylon 0.2 | Nylon 6,6 | neutral/amphoteric | 150 ±20 | 500 | 0,2/0,45 | UV, baking |
| Roti® Nylon plus | Nylon 6,6 | hydrophilic, positively charged | 160 ±20 | 500 | 0,45 | not necessary, UV and baking possible |
| Transfer membrane ROTI®NC 0.2 | Nitrocellulose | hydrophobic | 150 ±50 | 100 | 0,2 | UV, baking (max. 80 °C) |
| Transfer membrane ROTI®NC 0.45 | Nitrocellulose | hydrophobic | 150 ±50 | 100 | 0,45 | UV, baking (max. 80 °C) |
| Amersham Protran® | Nitrocellulose | electrostatic, hydrophobic | unknown | 11-72 | 0,2/0,45 | UV, baking (max. 80 °C) |
| Amersham Protran® supported | Nitrocellulose | electrostatic, hydrophobic | unknown | 86-110 | 0,2/0,45 | UV, baking (max. 80 °C) |
| Biomolecules / Membrane Pores | Size (kD) |
| genomic DNA (human) | >11000000 |
| E. coli | approx. 1500000 |
| 0.45 μm | approx. 5000 |
| 0.2 μm | approx. 2000 |
| DNA 3 kb | approx. 1000 |
| RNA 1 kb | approx. 300 |
| Antibodies (IgG) | approx. 150 |
| Proteins in the lab | approx. 10–300 |
| BSA (Albumin) | 69 |
| Biomolecules | Nylon plus | Nylon 0.2 | PVDF 0.2 | PVDF | Fluoro PVDF | NC | NC 0.45 |
| DNA | +++ | + | - | - | - | + | + |
| RNA | + | +++ | - | - | - | + | + |
| Proteins | - | + | +++ | +++ | +++ | + | + |
| Detection System | |||||||
| Colour | +++ | +++ | +++ | +++ | +++ | +++ | +++ |
| Radioactivity | +++ | +++ | +++ | +++ | +++ | +++ | +++ |
| Chemoluminescence | +++ | +++ | +++ | +++ | - | + | + |
| Fluorescence | +++ | +++ | - | - | +++ | - | - |
| +++: recommended | +: suitable | -: not | |||||