Ponceau S (C.I. 27195), 25 g
Molar mass (M) 760,56 g/mol
CAS No. [6226-79-5]
Suitable for NC and PVDF membranes as well as prior to Western analyses.
excl. VAT. | 25 g per Pack Qty.
Art. No. 5938.1
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Ponceau S (C.I. 27195) for histology and electrophoresis
Staining of NC membranes: 0.2-2 % Ponceau S in 3 % trichloroacetic acid (Art. No. 8789.1) + 3 % sulphosalicylic acid (Art. No. 4119.1), incubate for 1-5 mins. at room temperature. Enhance contrast by incubation in water acidified by a few drops acetic acid.
Staining of PVDF membranes: 0.1-1 % Ponceau S in 10 % acetic acid (Art. No. 6755.1), incubate 1-5 mins. at room temperature. Ponceau S stained bands on PVDF only appear bright and distinct after drying (not recommended if Western blotting shall be performed).
Prior to Western blot analysis decolourise in PBS (Art. No. 1058.1) or TBS (Art. No. 1060) for 10-20 mins. at room temperature.
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|Absorption max. (in H2O)||517-523 nm|
|Specific extinction E 1 %/1 cm||>460|
|Loss on drying (110 °C)||≤10 %|