Technical Data Sheet
Ponceau S (C.I. 27195), 25 g
Molar mass (M) 760,56 g/mol
WGK 1
CAS No. 6226-79-5
EG-Nr. 228-319-2
Suitable for NC and PVDF membranes as well as prior to Western analyses.
€67.65/Pack Qty.
excl. VAT. | 25 g per Pack Qty.
Art. No. 5938.1
Fits to / Accessories
Product details
Ponceau S (C.I. 27195) for histology and electrophoresis
Staining of NC membranes: 0.2-2 % Ponceau S in 3 % trichloroacetic acid (Art. No. 8789.1) + 3 % sulphosalicylic acid (Art. No. 4119.1), incubate for 1-5 mins. at room temperature. Enhance contrast by incubation in water acidified by a few drops acetic acid.
Staining of PVDF membranes: 0.1-1 % Ponceau S in 10 % acetic acid (Art. No. 6755.1), incubate 1-5 mins. at room temperature. Suitable for rapid control of transfer in Western blot, PVDF membrane should not be completely dried before immunodetection.
Prior to Western blot analysis decolourise in PBS (Art. No. 1058.1) or TBS (Art. No. 1060) for 10-20 mins. at room temperature.
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| Art. No. | Pack Qty. | Pack. | Price | Quantity | |
|---|---|---|---|---|---|
| 5938.1 | 25 g | glass |
€67.65 |
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| 5938.2 | 10 g | glass |
€33.25 |
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Downloads / MSDS
General information
Certificates of Analysis
Type analysis
| Absorption max. (in H2O) | 517-523 nm |
| Specific extinction E 1 %/1 cm | >460 |
| Loss on drying (110 °C) | ≤10 % |