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ROTI®Blot A

10x conc., for electrophoresis
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Examples of effect: Damage metals and burn body tissues; may cause serious eye damage. Safety: Avoid contact; wear safety spectacles and gloves. In the event of contact with eyes and skin, rinse with water.
Warning
H290-H315-H319
i may be corrosive to metals, causes skin irritation, causes serious eye irritation
P280 P302+P352 P305+P351+P338
i wear protective gloves/eye protection, IF ON SKIN: Wash with plenty of water, IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing
Pack Qty.
Pack.
Blotting buffers
Density (D) 1,035 g/cm³
Storage temp. +15 to +25 °C
Transport temp. ambient temp.
WGK 1

Anode buffer to Roti®-Blot 1 (L509)

Discontinuous buffer system for optimum transfer in the semi-dry blotter.
Product details

€80.55/Pack Qty. 

excl. VAT. | 1 l per Pack Qty.

Art. No. L510.1

In stock
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from 6 Pack Qty. €76.52/Pack Qty.
from 24 Pack Qty. €72.50/Pack Qty.

Product details


Discontinuous buffer system for optimum transfer in the semi-dry blotter.

  • Optimised for semi dry blotting
  • Superior blotting results for peptides and proteins of every size
  • Special formulation with reduced acid formation - for optimal electrode protection

In the semi-dry blotting system, both a continuous buffer system (identical buffer at anode and cathode) and a discontinuous buffer system (different buffer at anode and cathode) can be used. The transfer performance of discontinuous systems is, however, generally higher, since the two buffers are designed separately according to the needs of the two electrodes.

The discontinuous buffer system ROTI®Blot 1 consists of two different buffers, the anode buffer A (pH 7.8 ±0.1) and the cathode buffer K (pH 8.5 ±0.1). ROTI®Blot 1 shows a much better transfer efficiency than a tris-glycine buffer and can be used for proteins and peptides of any length.


Directions for use

The blot stack is built up by soaking the upper blotting papers with cathode buffer and the lower blotting papers with anode buffer. With slightly longer transfer times, proteins >100 kDa can also be completely transferred with ROTI®Blot 1.

A detailed instruction manual is enclosed with the product.



ROTI®Blot A 10x conc., for electrophoresis

ROTI®Blot A
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L510.1 1 l glass

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Downloads / MSDS


General information


Buffer Recommendations for Transfer (Blotting)

Buffers recommended for Tank blotting:
Acc. to Tobwin et al., 1997: 25 mM Tris, 192 mM glycin, 20 % methanol. Optional: 0-0,1 % SDS.
Acc. to Bjerrum und Schaefer-Nielsen, 1986: 48 mM Tris, 39 mM glycin, 0-20 % methanol. Optional: 0-0.1 % SDS.
Acc. to Dunn, 1986: 10 mM NaHCO3, 3 mM NaCO3, 20 % methanol.

Buffers recommended for Semy Dry blotting:
Discontinuous buffer system: ROTI®Blot 1 - for standard proteins or ROTI®Blot 2 - for hydrophobic proteins.
Continuous Tris-glycin buffer acc. to Bjerrum and Schaefer-Nielsen, 1986: 48 mM Tris, 39 mM glycin, 0-20 % methanol. Optional: 0,01-0,1 % (usually 0,0375 %) SDS.
Continuous CAPS buffer for blotting of basic proteins and prior to N-terminal sequencing: 0,22 % CAPS (pH 10,5-11,0), 10 % methanol.
Do not adjust pH of the buffer (exception: CAPS stock solution). Adding acid or base to the buffer will result in higher conductivity, leading to increased formation of heat as well as ions. Subsequently, this results in brown, “burnt” looking blotting paper and damage to the electrode plates.

Methanol prevents the gel from swelling during transfer (mandatory for gradient gels!) and improves the absorption of proteins to NC membranes. For transfer of native or big proteins, reduce or omit methanol.

SDS improves transfer efficiency of large proteins and protein binding to PVDF membranes, however increasing relative current, power and temperature. Don’t use SDS for Semi-Dry blotting of small proteins to NC membranes. For tank blotting use 0,02 % SDS in minimum.

For blotting of native proteins use buffer acc to Bjerrum and Schaefer-Nielsen with 0,04 % SDS and 0-10 % methanol.


Certificates of Analysis

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