Technical Data Sheet
ROTI®Cell Insulin solution
Boiling point (bp) 100 °C
Storage temp. +2 to +8 °C
Transport temp. ambient temp.
For cell culture
€75.15/Pack Qty.
excl. VAT. | 5 ml per Pack Qty.
Art. No. 3H70.1
Product details
ROTI®Cell Insulin solution CELLPURE® 5 mg/ml, sterile, human recombinant
Recombinant human insulin is commonly used as a chemical supplement for the cultivation of a variety of mammalian cell lines, particularly CHO cells. It regulates the cellular uptake, utilization, and storage of glucose, amino acids, and fatty acids, and also inhibits the breakdown of glycogen, proteins, and fats.
Expressed in yeast (Saccharomyces cerevisiae).
Instructions for use, addition | Store at +2 °C to +8 °C protected from light. Once opened, use within 4 weeks. |
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Art. No. | Pack Qty. | Pack. | Price | Quantity | |
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3H70.1 | 5 ml | plastic |
€75.15 |
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In stock
Available (note delivery time)
In procurement
No longer available
Delivery date currently unknown
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- Subtotal: 0.00
Downloads / MSDS
General information
In addition to the various nutrients in the medium, the pH value also plays a very important role in cell culture. The physiological pH for almost all mammalian cells is 7.4. Deviating from this, it has been observed that certain transformed cell lines prefer a pH between 7.0 and 7.4 and certain fibroblasts prefer a pH between 7.4 and 7.7. In any case, pH fluctuations should be avoided and the pH should be maintained as well as possible. That is why a buffer system in media is necessary. The most important buffer system in the body is the carbonic acid-carbonate buffer system. Accordingly, almost all cell culture media contain bicarbonate (NaHCO3). Bicarbonate alone leads to a strong pH increase of the medium, so that NaHCO3-buffered media must always be used with CO2 incubators. Depending on the NaHCO3 concentration in the medium, different CO2 environments are necessary to ensure a pH of about 7.4. For this reason, you should not work outside of the CO2 incubator for too long. If this is necessary, a HEPES buffer should be used in addition to the NaHCO3, which contributes as a second buffer system to stabilize the pH value.
Certificates of Analysis
Type analysis
pH value | 7.5-8.4 |
Osmolality | ≤20 mOsm/kg |
Endotoxins | ≤10 EU/ml |
Sterility | complies |