Technical Data Sheet
ROTI®Cell Insulin-Transferrin-Selenium (ITS) solution
Sterile products
Boiling point (bp) 100 °C
Storage temp. +2 to +8 °C
Transport temp. ambient temp.
For cell culture
€51.50/Pack Qty.
excl. VAT. | 10 ml per Pack Qty.
Art. No. 3H71.1
Product details
ROTI®Cell Insulin-Transferrin-Selenium (ITS) solution CELLPURE® 100x in EBSS, sterile
ITS (Insulin, Transferrin, Selenium) is a proven combination of growth factors for serum-free cell culture. This chemically defined supplement reduces the required amount of animal components, such as FBS, in basal media. It is also routinely used in serum-free media to enhance cell viability and proliferation.
Human recombinant insulin: 1 000 mg/L
Human transferrin: 550 mg/L
Sodium selenite: 0.68 mg/L
It is a 100x concentrated solution in Earle’s Balanced Salt Solution (EBSS).
| Instructions for use, addition | Store at +2 °C to +8 °C protected from light. Once opened, use within 4 weeks. |
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| Art. No. | Pack Qty. | Pack. | Price | Quantity | |
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| 3H71.1 | 10 ml | plastic |
€51.50 |
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In stock
Available (note delivery time)
In procurement
No longer available
Delivery date currently unknown
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- Subtotal: 0.00
Downloads / MSDS
General information
In addition to the various nutrients in the medium, the pH value also plays a very important role in cell culture. The physiological pH for almost all mammalian cells is 7.4. Deviating from this, it has been observed that certain transformed cell lines prefer a pH between 7.0 and 7.4 and certain fibroblasts prefer a pH between 7.4 and 7.7. In any case, pH fluctuations should be avoided and the pH should be maintained as well as possible. That is why a buffer system in media is necessary. The most important buffer system in the body is the carbonic acid-carbonate buffer system. Accordingly, almost all cell culture media contain bicarbonate (NaHCO3). Bicarbonate alone leads to a strong pH increase of the medium, so that NaHCO3-buffered media must always be used with CO2 incubators. Depending on the NaHCO3 concentration in the medium, different CO2 environments are necessary to ensure a pH of about 7.4. For this reason, you should not work outside of the CO2 incubator for too long. If this is necessary, a HEPES buffer should be used in addition to the NaHCO3, which contributes as a second buffer system to stabilize the pH value.
Certificates of Analysis
Type analysis
| pH value | 6.8-7.8 |
| Osmolality | 240-300 mOsm/kg |
| Endotoxins | ≤1 EU/ml |
| Sterility | complies |