Technical Data Sheet
ROTI®Cell Trypsin solution (1x)
All products identified as being particularly temperaturesensitive are shipped in special ice boxes with freezer packs or in dry ice.
Sterile products
Ready-to-use products and reagents
Boiling point (bp) ~100 °C
Storage temp. -20 °C
Transport temp. cooled
Thawing should be done in a +37 °C water bath or overnight at +2 °C to +8 °C. Swirl gently to homogenize.
As an alternative to Trypsin, the morecell-protective solution ROTI®Cell Accutase solution can be used, which is free of mammalian components.
€20.35/Pack Qty.
excl. VAT. | 100 ml per Pack Qty.
Art. No. 1Y17.1
Alternative products
Product details
Trypsin is a protein-cleaving digestive enzyme of the pancreas belonging to the serine proteases, which cleaves lysine and arginine at the C-terminus. Trypsin solutions are used for the separation of cells from tissue or cell monolayers. Thus, they are essential for routine applications in cell culture passaging, for cell culture experiments to obtain an adequate number of cells or for dissociation of primary tissue.
As an alternative to Trypsin, the morecell-protective solution ROTI®Cell Accutase solution can be used, which is free of mammalian components.
ROTI®Cell Trypsin solution (1x) CELLPURE® 0.25 % in DPBS, ready-to-use, sterile
The required trypsin concentration depends primarily on the cell type and the age of the cell culture. For orientation, you will find a complete protocol in the technical info brochure.
After cell dissociation, trypsin should be inhibited. This can be done either by using FCS or trypsin inhibitors.
| Enzyme | Trypsin from porcine pancreas |
| Alternative Name | Cell dissociation reagents |
| Use | Cell dissociation |
| Instructions for use, addition | Preparation of aliquots recommended to avoid repeated freeze-thaw cycles. |
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| Art. No. | Pack Qty. | Pack. | Price | Quantity | |
|---|---|---|---|---|---|
| 1Y17.1 | 100 ml | plastic |
€20.35 |
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No longer available
Delivery date currently unknown
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Downloads / MSDS
Certificates of Analysis
Type analysis
| pH value | 7.0-7.6 |
| Osmolality | 260-340 mOsm/kg |
| Endotoxins | ≤10.0 EU/ml |
| Sterility | complies |