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Vitexin

ROTICHROM® TLC
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Orientosid, Apigenin-8-glucoside
Empirical formula C21H20O10
Molar mass (M) 432,36 g/mol
Melting point (mp) 265 °C
Storage temp. +4 °C
WGK 1
CAS No. 3681-93-4
EG-Nr. 222-963-8

Reference substance for thin layer chromatography.

€51.50/Pack Qty. 

excl. VAT. | 10 mg per Pack Qty.

Art. No. 5579.1

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Downloads / MSDS


General information

Further attractive products to complete your chromatography laboratory can be found on our Chromatography page!


Thin Layer Chromatography (TLC)

Although the principle of thin-layer chromatography is more than a century old, it did not make its breakthrough as an analytical method until about 50 years ago.
Thanks to the development of new sorbents and supports, as well as increasing instrumentation and automation, TLC has become a versatile separation method. It is used both in qualitative analysis and in quantitative analysis.
Applications range from simple manual separation processes in classic TLC to automated processes in HPTLC (high performance thin layer chromatography).

Advantages of thin layer chromatography:

  • Higher sample throughput in less time
  • Suitable for screening tests
  • Pilot process for HPLC
  • The ready-to-use TLC layer functions as a data storage device for separation results
  • The separated substances can be used later for further analysis (e.g. IR, MS)
  • By switching the mobile and the stationary phases, the separation process can be optimised quickly and cost-efficient


For reference substances

  • ROTICHROM® HPLC
  • ROTICHROM® TLC
  • ROTICHROM® GC
  • a batch-specific
      HPLC-chromatogram, TLC-chromatogram and GC-chromatogram respectively
    is prepared, which you can find in the respective certificate of analysis. The batch-related certificate of analysis with chromatogram is available online.
      Reference substances
  • ROTICHROM® CHR
  • are examined chromatographically. The respective chromatograms are not included in the certificate of analysis.


    Reference Substances for Chromatography

    ROTICHROM® HPLC/TLC/GC

    Pure reference substances are essential for precise analysis. They are used for identifying compounds by retention time, for calibrating detectors, and for eliminating errors in quantification of the sample compounds.

    Peak areas are proportional to sample quantity in all chromatographic methods. For precise quantitative analysis, however, it is necessary to calibrate the detector as the signal may significantly vary with different substances.

    To avoid errors during sample preparation and injection, an internal standard should be used in any case. The standard must not be present in the sample, however, its chemical and physical properties ought to be the closest possible to the analytical targets. It must be well resolved in the chromatogram within the same range of retention time as the sample. Before sample analysis, a given volume of standard is added, and all clean-up procedures are made with the sample containing the standard. Quantitative errors may be compensated by comparing the peak-areas of the sample with that of the internal standard.


    Reference Substances for TLC

    ROTICHROM® TLC et CHRTLC

    ROTICHROM® TLC reference substances show the description of the chromatographic method in the certificate of analysis.A compound in this catalogue is designated as beingthin-layer chromatographically pure (CHRTLC) if noimpurities can be detected during test conditionswith 20 μg sample.For products with impurities which under ourconditions, (CHRTLC), allow the main compoundto be designated; such products can be identifiedas being “almost thin-layer chromatographicallypure” (~CHRTLC).


    Certificates of Analysis

    You can search for and download your certificate of analysis for the selected product here. Please provide your batch number.
    The following analysis certificates have been found: