Natural product reagent A, 25 g
Molar mass (M) 225,10 g/mol
Density (D) 1,2 g/cm³
Melting point (mp) 192 °C
Storage temp. +4 °C
CAS No. [524-95-8]
Application: Natural substance-polyethylene glycol reagent (NST/PEG). Spray a 1 % methanol solution of natural substance reagent A and a 5 % ethanol PEG 4000 solution, one after the other onto the TLC-plate (approx. 10 ml and 8 ml). Intensive fluorescent colours will appear immediately or after 15 min in UV-365 nm. Adding PEG will lead to an increase in detection sensitivity (from 10 µg to 2.5 µg). The fluorescence behaviour depends on the structure. The reagent must be freshly prepared before use (1 % in methanol). Spray under the fume hood!
excl. VAT. | 25 g per Pack Qty.
Art. No. 9920.2
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Natural product reagent A ≥98 %, p.a.
Natural product reagent A as a spray reagent: According to Wagner natural substance A is an excellent reagent for fluorometry in quantitative analysis of thin layer chomatogrammes.
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Although the principle of thin-layer chromatography is more than a century old, it did not make its breakthrough as an analytical method until about 50 years ago.
Thanks to the development of new sorbents and supports, as well as increasing instrumentation and automation, TLC has become a versatile separation method. It is used both in qualitative analysis and in quantitative analysis.
Applications range from simple manual separation processes in classic TLC to automated processes in HPTLC (high performance thin layer chromatography).
Advantages of thin layer chromatography:
- Higher sample throughput in less time
- Suitable for screening tests
- Pilot process for HPLC
- The ready-to-use TLC layer functions as a data storage device for separation results
- The separated substances can be used later for further analysis (e.g. IR, MS)
- By switching the mobile and the stationary phases, the separation process can be optimised quickly and cost-efficient
|Appearance||white cryst. powder|
|Melting point||190-194 °C|