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Methylene blue (C. I. 52015), 10 g, glass

pure
Examples of effect: Lead to damage to heath, cause irritation to eyes, skin or respiratory organs. Are fatal in larger quantities. Safety: As previously stated, in the event of skin irritation or contact with eyes, rinse with water or a suitable medium.
Warning
H302
i harmful if swallowed
P270 P301+P312
i do not eat, drink or smoke when using this product, IF SWALLOWED: Call a POISON CENTER/doctor if you feel unwell
Pack Qty.
Pack.
Basic blue 9
Empirical formula C16H18ClN3S
Molar mass (M) 319,9 g/mol
Melting point (mp) 190 °C
WGK 1
CAS No. 61-73-4
EG-Nr. 200-515-2

For microscopy and staining of nucleic acids in polyacrylamide gels.
Dissolve 0.1-0.5 g in 100 ml of water

Watersoluble nuclear dye, does not intercalate between base pair stacks of DNA. Especially used for staining of blood cells.

Application note:

Staining of nucleic acids in PAA

Nucleic acids are visible as blue bands.

Detection limit: 20 ng/band.
Product details

€24.20/Pack Qty. 

excl. VAT. | 10 g per Pack Qty.

Art. No. A514.1

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Product details



Methylene blue (C. I. 52015) pure

Watersoluble nuclear dye, does not intercalate between base pair stacks of DNA. Especially used for staining of blood cells.

Application note:

Staining of nucleic acids in PAA

Nucleic acids are visible as blue bands.

Detection limit: 20 ng/band.

  • Use a 0.2 % solution of methylene blue for staining
  • Stain for 20 min.
  • Wash for 15 min. in distilled water


Technical Information
Dyeing dead cells blue 
Ox. colour blue 
Red. colour colourless 
Redox potential E0[V] at pH=0 and 20 °C +0.53 (30 °C) 
Use vitality test 
Methylene blue (C. I. 52015)
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Art. No. Pack Qty. Pack. Price Quantity
A514.1 10 g glass

€24.20

A514.2 50 g glass

€64.40

A514.3 100 g glass

€110.20

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Downloads / MSDS


General information

Determination of Surfactants

Benzethonium chloride solution is used to measure anionic detergents. In this method, the anionic detergent and the cationic detergent form a 1:1 salt in dichloromethane. The end point of the titration is indicated by methylene blue.


Indicators and Dyes

Carl ROTH offers many indicators and dyes of high purity, which can be used in special applications.


Certificates of Analysis

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The following analysis certificates have been found:

Type analysis

AppearanceDark green crystalline powder
Assay (dry substance)≥95 %
Maximum absorption655-670 nm
Loss on drying (1 h, 100 °C)≤10 %