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Dialysis membrane ZelluTrans/ROTH T4: MWCO 12,000–14,000, 25 mm

From  ROTH SELECTION
Icon_Autoklavierbar All autoclavable products in our catalogue are identified by this symbol. Please also note any additional instructions when autoclaving.
Material: cellulose, regenerated. Autoclavable: yes.
Related products
MWCO
Surface width
Packaging


€164.50/Pack Qty. 

excl. VAT. | 1 unit(s) per Pack Qty.

Art. No. E683.1

Not available
from 6 Pack Qty. €156.28/Pack Qty.
from 24 Pack Qty. €148.05/Pack Qty.
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Product details

Material: cellulose, regenerated. Autoclavable: yes.

  • High-quality membranes on rolls, packaged dry
  • Treated with glycerine to protect against embrittlement
  • Very low membrane adsorption (e.g. 1 µg/cm2 bovine serum albumin)
  • Max. application temperature +90 °C
  • Good resistance to chemicals
  • Contain low quantities of trace elements, sulphides and heavy metals
  • Wall thickness: 20–50 µm
  • Autoclavable up to 121 °C (max. 10 min at 1 bar, immersed in distilled water)
  • Metal traces: Cadmium < 0–10 ppm, nickel < 0.10 ppm, chromium < 0.10 ppm, zinc 6.24–12.9 ppm, copper < 0.10 ppm, lead 0.75–0.80 ppm, iron < 13.6 ppm


Dialysis membrane ZelluTrans/ROTH T4: MWCO 12,000–14,000 

Not a medical device / Not an IVD product
Delivery incl. detailed operating manual, single-use scissors and 2 locking clips.
Technical Information
MWCO 12000–14000 dalton(s)
Surface width 25 mm
Ø 16 mm
Wall thickness 20–50 µm
Packaging type Roll 
Dialysis membrane ZelluTrans/ROTH T4: MWCO 12,000–14,000
Selected quantity:   0
  1. Subtotal:  0.00
Order No. MWCO Surface width Packaging Pack Qty. Price Quantity
E685.1 12000–14000 Dalton 45 mm 30 m 1 unit(s)

€192.45

E682.1 12000–14000 Dalton 10 mm 30 m 1 unit(s)

€359.05

E684.1 12000–14000 Dalton 33 mm 30 m 1 unit(s)

€170.95

E683.1 12000–14000 Dalton 25 mm 30 m 1 unit(s)

€164.50

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Selected quantity:   0
  1. Subtotal:  0.00

Downloads / MSDS

No documents available

General information

Microdialysis by Membrane Filter (Drop Dialysis)

Microvolumes of sample (5-100 μl) can also be dialysed by membrane filter discs made of cellulose mixed ester (pore size 0,025 μm, Art. No. CY35.1-CY37.1): Fill a Petri dish with dialysis buffer. Place the membrane filter disc on the surface of the solution (shiny side up, avoid bubbles under the filter) by using a stainless steel forceps. Float the membrane for ca. 5 min until it is completely soaked. Pipette the sample on the center of the filter. Due to the open pore structure of the membrane filter and the high surface-to-volume ratio the dialysis is done within a short time (mostly < 30 min). To avoid evaporation of the sample seal the Petri dish tightly during the procedure. In this way the sample volume remains nearly constant.


Filling volume for 15 cm tube length

Broadth (mm) 8 10 12 18 25 32 40 45 50 76 100 120
Tube Ø (mm) 5 6 8 11 16 20 25 29 32 48 64 76
Filling volume (ml) 3,1 4,8 7 15 30 49 76 97 119 276 478 688

Attention:

Do not tie knots in regenerated cellulose. Closure clips must be used to avoid any changing of membrane or MWCO.


Appearance of dialysis membranes

Dry dialysis membranes have different appearances, dependent on pore size and wall thickness: Clear, opaque or even completely white.

Reason:

Larger MWCO membranes are made slightly thicker to maintain membrane strength when the pores are larger.

The thicker the membrane and larger the pores, the more opaque. So the different membranes look as follows:

Dry membranes with MWCO

100-1000: clear

3 500-5 000: mostly clear

8 000-10 000: slightly opaque

20 000-50 000: opaque

≥100 000: white.

When the membrane is wetted with water the effect dissapears and the membrane becomes more clear.


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