Dialysis membrane ZelluTrans/ROTH T4: MWCO 12,000–14,000, 25 mm
excl. VAT. | 1 unit(s) per Pack Qty.
Art. No. E683.1
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- High-quality membranes on rolls, packaged dry
- Treated with glycerine to protect against embrittlement
- Very low membrane adsorption (e.g. 1 µg/cm2 bovine serum albumin)
- Max. application temperature +90 °C
- Good resistance to chemicals
- Contain low quantities of trace elements, sulphides and heavy metals
- Wall thickness: 20–50 µm
- Autoclavable up to 121 °C (max. 10 min at 1 bar, immersed in distilled water)
- Metal traces: Cadmium < 0–10 ppm, nickel < 0.10 ppm, chromium < 0.10 ppm, zinc 6.24–12.9 ppm, copper < 0.10 ppm, lead 0.75–0.80 ppm, iron < 13.6 ppm
Dialysis membrane ZelluTrans/ROTH T4: MWCO 12,000–14,000
|Surface width||25 mm|
|Wall thickness||20–50 µm|
- Subtotal: 0.00
|Order No.||MWCO||Surface width||Packaging||Pack Qty.||Price||Quantity|
|E685.1||12000–14000 Dalton||45 mm||30 m||1 unit(s)||
|E682.1||12000–14000 Dalton||10 mm||30 m||1 unit(s)||
|E684.1||12000–14000 Dalton||33 mm||30 m||1 unit(s)||
|E683.1||12000–14000 Dalton||25 mm||30 m||1 unit(s)||
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Microvolumes of sample (5-100 μl) can also be dialysed by membrane filter discs made of cellulose mixed ester (pore size 0,025 μm, Art. No. CY35.1-CY37.1): Fill a Petri dish with dialysis buffer. Place the membrane filter disc on the surface of the solution (shiny side up, avoid bubbles under the filter) by using a stainless steel forceps. Float the membrane for ca. 5 min until it is completely soaked. Pipette the sample on the center of the filter. Due to the open pore structure of the membrane filter and the high surface-to-volume ratio the dialysis is done within a short time (mostly < 30 min). To avoid evaporation of the sample seal the Petri dish tightly during the procedure. In this way the sample volume remains nearly constant.
|Tube Ø (mm)||5||6||8||11||16||20||25||29||32||48||64||76|
|Filling volume (ml)||3,1||4,8||7||15||30||49||76||97||119||276||478||688|
Do not tie knots in regenerated cellulose. Closure clips must be used to avoid any changing of membrane or MWCO.
Dry dialysis membranes have different appearances, dependent on pore size and wall thickness: Clear, opaque or even completely white.
Larger MWCO membranes are made slightly thicker to maintain membrane strength when the pores are larger.
The thicker the membrane and larger the pores, the more opaque. So the different membranes look as follows:
Dry membranes with MWCO
3 500-5 000: mostly clear
8 000-10 000: slightly opaque
20 000-50 000: opaque
≥100 000: white.
When the membrane is wetted with water the effect dissapears and the membrane becomes more clear.