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ROTI®Pol Hot-TaqS Red-Mix (2x), 2.5 ml, 1 x 2,5 ml

2x konz., ready-to-use
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Pack Qty.
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Packaging
PCR master mix
Density (D) 1 g/cm³
Storage temp. -20 °C
Transport temp. cooled
WGK 1

Taq master mix for hot start PCR amplifications followed by direct gel-loading.
Prestained PCR premix w/o primers and template, optimized for reactions of 25 µl volume.

For PCR. Recombinant, heat stable DNA polymerases from the thermophilic bacteria Thermus aquaticu ...
Product details
Type analysis

€69.80/Pack Qty. 

excl. VAT. | 2.5 ml per Pack Qty.

Art. No. 9256.1

In stock
24 hour direct shipment
from 6 Pack Qty. €66.31/Pack Qty.
from 24 Pack Qty. €62.82/Pack Qty.
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Product details


For PCR. Recombinant, heat stable DNA polymerases from the thermophilic bacteria Thermus aquaticus.


ROTI®Pol, the series of DNA polymerases is the optimal choice for all PCR cycling protocols, as being performed in, for instance, analysis of cloning efficiency, for gene fishing, in routine screening processes, educational assays and much more. In combination with our specially designed buffers, the ROTI®Pol DNA polymerases deliver specific and reproducible PCR amplification with a wide range of PCR templates.



ROTI®Pol Hot-TaqS Red-Mix (2x) 2x konz., ready-to-use

Optimized pre-mixed 2x PCR solution containing the recombinant Hot-TaqS DNA polymerase from the thermophilic bacterium Thermus aquaticus, dNTPs, MgCl2, and all other components required for PCR (w/o primers and template DNA), plus components for direct gel electrophoresis. ROTI®Pol Hot-TaqS Red-Mix (2x) is recommended for use in highly specific PCR applications immediately followed by gel electrophoresis.

PCR assaying with ROTI®Pol Hot-TaqS Red-Mix (2x) master mix not only reduces contamination risks, but is also time-saving, highly reproducible and very easy to prepare. ROTI®Pol Hot-TaqS Red-Mix (2x) is superior for use in all standard Taq-based cycling protocols when big sample numbers shall be amplified with high specificity as well as high reproducibility. ROTI®Pol Hot-TaqS Mix (2x) master mix is, therefore, the optimal choice for high throughput PCR assays in colony screening, prior to sequencing, in cycle sequencing, and in similar assays.

Due to the optimized composition of the master mix combined with the antibody-blocked Hot-TaqS polymerase, ROTI®Pol Hot-TaqS Red-Mix (2x) delivers highly specific PCR amplification of good yield with a wide range of PCR templates. The antibody-mediated blocking of the DNA polymerase is released only at the initial denaturation step, hence resulting in highly specific amplification of the target sequence without production of unwanted side products caused by unspecific primer annealing.

ROTI®Pol Hot-TaqS Red-Mix (2x) is able to amplify PCR products up to 3 kb with genomic DNA and up to 5 kb with Lambda DNA, and is appropriate for use with pure DNA solutions, cDNA, and bacterial colonies as templates. In 1 % agarose gels, the included red dye migrates approx. as fast as a 1 kb DNA fragment. During denaturation in Southern blotting, the dye turns yellow at an acidic pH. The Hot-TaqS polymerase included in the master mix possesses a 5’ → 3’ polymerase- as well as a 5’-flap endonuclease activity, and generates a 3’dA (adenine)-overhang which may well be used for TA-cloning purposes.

RotiPol-Sensitivitaetsassay
Figure: Sensitivity assay using ROTI®Pol TaqS, TaqHY, Hot-TaqS and Hot-TaqHY, 1 U/reaction each (20 μl). 300 bp β-Actin fragment, 40 cycles. Template: 10 to 2000 pg human gDNA. Gel loading 10 μl each.
M: 100 bp-DNA Ladder extended. NTC: no template control.

2 or 10 vials, respectively, with 1,25 ml each of ROTI®Pol Hot-TaqS Red-Mix (2x) containing Hot-TaqS polymerase, 0,4 mM each dNTP, and 4 mM MgCl2 in 2x reaction buffer with 0,02 % cresol red.



Technical Information
Use Particularly sequence specific standard PCR with subsequent gel loading  
Amplicon ends 3'dA  
Master-Mix red (ready to load)  
Polymerase Hot start Taq polymerase  
Use Particularly sequence specific standard PCR with subsequent gel loading  
ROTI®Pol Hot-TaqS Red-Mix (2x)
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Order No. Pack Qty. Pack. Packaging Price Quantity
9256.1 2.5 ml plastic 1 x 2,5 ml €69.80
9256.2 12.5 ml plastic 5 x 2,5 ml €321.45
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Downloads


Analysis certificates

You can search for and download your certificate of analysis for the selected product here. Please provide your batch number.
The following analysis certificates have been found:

Type analysis

Appearancered
Endonuclease activitynone detected
Exonuclease activitynone detected
Suitability for PCR (400 bp)complies
Suitability for PCR (3 kb)complies
Suitability for PCR (5 kb)complies
Selective specificity in PCRcomplies
For research use only. Not approved for use in clinical or in vitro diagnostics.