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ROTI®Pol Hot-TaqUltra, 40 µl, 1 x 200 U

5 U/μl, DNA-free
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DNA polymerase (thermostable), Hot start DNA polymerase, Taq DNA polymerase, recombinant, Polymerase (thermostable)
Storage temp. -20 °C
Transport temp. cooled
WGK 1

For hot start PCR amplifications, particularly of bacterial DNA.
Ultra pure HotTaq polymerase in storage buffer

For PCR. Recombinant, heat stable DNA polymerases from the thermophilic bacteria Thermus aquaticu ...
Product details
Type analysis

€155.90/Pack Qty. 

excl. VAT. | 40 µl per Pack Qty.

Art. No. 9350.1

Available short-term
from 6 Pack Qty. €148.10/Pack Qty.
from 24 Pack Qty. €140.31/Pack Qty.
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Product details


For PCR. Recombinant, heat stable DNA polymerases from the thermophilic bacteria Thermus aquaticus.


ROTI®Pol, the series of DNA polymerases is the optimal choice for all PCR cycling protocols, as being performed in, for instance, analysis of cloning efficiency, for gene fishing, in routine screening processes, educational assays and much more. In combination with our specially designed buffers, the ROTI®Pol DNA polymerases deliver specific and reproducible PCR amplification with a wide range of PCR templates.



ROTI®Pol Hot-TaqUltra 5 U/μl, DNA-free

Hot start version of the recombinant heat stable Taq DNA polymerase from the thermophilic bacterium Thermus aquaticus in storage buffer and tested on the absence of bacterial DNA. ROTI®Pol Hot-TaqUltra is recommended for use in highly specific PCR applications in general, or if bacterial DNA, or in RT-PCR, bacterial 16S rRNA shall be detected.
ROTI®Pol Hot-TaqUltra is purified using a multiple-step process that minimizes contaminating bacterial DNA to a none detectable level. Each lot of the polymerase undergoes strict quality control testing in order to ensure the absence of detectable amounts of contaminating bacterial DNA. ROTI®Pol Hot-TaqUltra is provided in a DNA-free storage buffer containing 50 % glycerol.
ROTI®Pol Hot-TaqUltra is recommended for use when particularly specific amplification of bacterial DNA is the main focus. The antibody-mediated blocking of the DNA polymerase is released only at the initial denaturation step, hence resulting in highly specific amplification of the target sequence without production of unwanted side products caused by unspecific primer annealing.
ROTI®Pol Hot-TaqUltra is able to amplify PCR products up to 5 kb and is appropriate for use in the amplification of DNA from eukaryotic as well as prokaryotic templates. The Hot-TaqUltra DNA polymerase possesses a 5’ → 3’ polymerase- as well as a 5’-3' exonuclease activity, and generates a 3'dA (adenine)-overhang which may well be used for TA-cloning purposes.



Technical Information
Use Particularly sequence specific PCR of bacterial gDNA  
Amplicon ends 3'dA  
Polymerase DNA free, ultra pure hot start Taq polymerase  
Use Particularly sequence specific PCR of bacterial gDNA  
ROTI®Pol Hot-TaqUltra
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Order No. Pack Qty. Pack. Packaging Price Quantity
9350.1 40 µl plastic 1 x 200 U €155.90
9350.2 200 µl plastic 5 x 200 U €740.70
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Downloads


Analysis certificates

You can search for and download your certificate of analysis for the selected product here. Please provide your batch number.
The following analysis certificates have been found:

Type analysis

Purity (enzyme)≥98%
Enzyme activity5 U/µl
Endonuclease activitynone detected
Exonuclease activitynone detected
Suitability for PCR (400 bp)complies
Suitability for PCR (3 kb)complies
Bacterial DNA (enzyme)none detected
Selective specificity in PCRcomplies
Hot start functionalitycomplies
Stability (in storage buffer)complies
For research use only. Not approved for use in clinical or in vitro diagnostics.