Cycloheximide, 5 g
Molar mass (M) 281,36 g/mol
Melting point (mp) 112 °C
Storage temp. +4 °C
ADR 6.1 II
CAS No. [66-81-9]
Stock solution: 10 mg/ml in H2O or acetone.
Storage of stock solution at -20 °C.
Working concentration: 1-10 µg/ml.
Note: Inactivated at basic pH values. Heat- and acid stabile. No inactivation after 15 mins. 95 °C in pH 7.0.
excl. VAT. | 5 g per Pack Qty.
Art. No. 8682.3
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Cycloheximide ≥98 %, for biochemistry
Antibiotic isolated from Streptomyces griseus. Its main biological activity is translation inhibition in eukaryotes by binding to the 60S subunit of the ribosomes. Cycloheximide inhibits binding of Aminoacyl-tRNA to the ribosomes, Peptidyltransferase, detachment of deacylated tRNA, and translocation of Aminoacyl-tRNA, resulting in cell growth arrest and apoptotic cell death.
Not effective against bacteria. Cycloheximide is widely used for selection of cycloheximide-resistant strains of yeast and fungi, for inhibition of eukaryotic contaminants in bacterial microbiology, for controlled inhibition of protein synthesis in cell culture and for detection of short-lived proteins, and for apoptosis induction or facilitation of apoptosis induction by death receptors.
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|Appearance||white to off-white powder|
|Assay (HPLC)||≥98 %|
|Water (KF)||≤0,5 %|
|Specific rotation [α]a (c=1 % in CHCl3)||-32° to -26°|
|Ash content||≤0,5 %|