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dNTP-Set 1 (pH 7), 5 x 4 x 25 μmol

≥99 %
Related products
Pack Qty.
Deoxynucleoside triphosphate set, Nucleotide set for PCR
Boiling point (bp) 100 °C
Storage temp. -20 °C
Transport temp. cooled

for PCR and RT, nucleotide set composed of solutions of dATP, dTTP, dGTP, dCTP, pH 7,0 ±0,1
Concentration: 100 mM per dNTP
Typical end concentration in PCR: 200 µM each

€421.95 /Pack Qty.

€295.35/Pack Qty.  Campaign price!

excl. VAT. | 1 set per Pack Qty.

Art. No. 0178.2

Available at short notice
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Product details

  • Available as ready-to-use set or mix, for contamination-free applications
  • Can be optimally combined with ROTI®Pol DNA polymerases from Carl ROTH
  • Tested for “long range PCR“
  • DNAse-, RNAse-, Protease- and Phosphatase-free
  • Free of PCR inhibitors like modified bases and tetra-pyrophosphate
  • Adjusted to pH 8,5 for superior stability even during larger numbers of freeze-and-thaw cycles
  • Also available adjusted to pH 7,0 for special applications
  • Highly efficient enzymatic fabrication of dATP, dGTP, dCTP and dUTP. dTTP ist synthesized chemically.

Suitable for

PCR, light-cycling, cDNA synthesis, labelling and primer-extension, mutagenesis-assays, sequencing reactions and in vitro transcription.

All Carl ROTH nucleotides are manufactured from highest-quality reagents and are most thoroughly tested for quality. This testing procedure not only includes standard-PCR but also ’long range PCR’, repeated quantitative light-cycling reactions, and tests for physical stability.

dNTP-Set 1 (pH 7) ≥99 %

Figure 1:
Essai de sensibilité. Amplification d'un fragment de 260 bp sur un modèle de (de g. à d.) 250 ng (A), 25 ng (B) et 2,5 ng (C) d'ADN génomique humain (2 préparations chaque fois).

Figure 2:
Sensitivity assay II: Quantitative light-cycling-PCR on recombinant DNA with (left to right) 10 ng, 1 ng, 100 pg, 10 pg, 1 pg DNA as template (six replicates).

Figure 3:
Stability testing: HPLC analysis of all four dNTPs following incubation periodes of 1–14 days at different temperatures (4 °C – green; room temperature – red; 40 °C – blue). Stability of nucleotides is >99 % even after 14 days incubation at room temperature. Even after an incubation period of 9 days at 40 °C, 85 % of all nucleotide molecules are still intact.

dNTP-Set 1 (pH 7)
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Order No. Pack Qty. Pack. Packaging Price Quantity
0178.1 1 set plastic 4 x 25 μmol €71.35
0178.2 1 set plastic 5 x 4 x 25 μmol €295.35
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