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Fluorescein-12-dUTP, 25 µl, 25 nmol

≥95 %, 1 mM solution
Icon_DNAse-free
Icon_RNAse-free
Icon_Protease-free
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Fluorescein-6-carboxyaminocaproyl-[5-(3-aminoallyl)-2'-deoxyuridine-5'-triphosphate]
Empirical formula C39H41N4O21P3
Molar mass (M) 994,66 g/mol
Boiling point (bp) 100 °C
Storage temp. -20 °C
Transport temp. cooled
WGK 1

For non-radioactive, enzymatic labelling of DNA.

€185.45/Pack Qty. 

excl. VAT. | 25 µl per Pack Qty.

Art. No. 1048.1

Available at short notice
from 6 Pack Qty. €176.18/Pack Qty.
from 24 Pack Qty. €166.90/Pack Qty.
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Product details


  • DNAse-, RNAse-, Protease- and Phosphatase-free
  • Free of PCR inhibitors like modified bases and tetra-pyrophosphate
  • Adjusted to pH 7,5 for optimzed enzyme compatibility
  • Highly efficient enzymatic fabrication
  • Can be optimally combined with Carl ROTH ROTI®Pol DNA polymerases

Suitable for

PCR, cDNA synthesis, labelling and primer-extension, mutagenesis-assays, sequencing reactions and in vitro transcription.


All Carl ROTH nucleotides are manufactured from highest-quality reagents and are most thoroughly tested for quality. This testing procedure not only includes standard-PCR but also ’long range PCR’, repeated quantitative light-cycling reactions, and tests for physical stability.



Fluorescein-12-dUTP ≥95 %, 1 mM solution

Fluorescein-12-dUTP is able to replace dTTP in growing DNA-strands and is used for efficient non-radioactive DNA-labelling. Detection of labelled nucleic acids can be done by direct analysis of fluorescent signals (e.g. by fluorescence microscopy) or by incubation with AP-coupled anti-fluorescein antibodies.
Fluorescein-12-dUTP can also be used for double staining techniques in combination with rhodamine-12-dUTP or biotin-11-dUTP and corresponding antibodies or streptavidin-complexes, respectively.

Excitation: 494 nm
Emission: 520 nm

1048
Figure:
Detection of chromosome wcp21q (whole chromosome paint, red arrows) and band 11q23 (BAC, bacterial artificial chromosome, white arrows) by FISH, labelling with fluorescein-12-dUTP.
With kind permission of PD Dr. Thomas Liehr, Institute of Human Genetics and Anthropology, Friedrich-Schiller University, Jena.

Applications: Non-radioactive labelling of DNA by enzymatic reactions, e.g. PCR, reverse transcription, nick-end-translation, end-labelling or random-primed DNA-labelling. Incorporation can be done with all established DNA-polymerases (e.g. Taq-polymerase, T4 DNA-polymerase, Klenow fragment).
Testet for the lack of endo-, exodeoxyribonuclease, ribonuclease and phosphatase.


ε495 (pH 9) = 70,0 E x mmol-1 x cm-1; pH: 7,5 ±0,2

Nukleotide_Stabilitaet
Figure 3:
Stability testing: HPLC analysis of all four dNTPs following incubation periodes of 1–14 days at different temperatures (4 °C – green; room temperature – red; 40 °C – blue). Stability of nucleotides is >99 % even after 14 days incubation at room temperature. Even after an incubation period of 9 days at 40 °C, 85 % of all nucleotide molecules are still intact.


Fluorescein-12-dUTP
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1048.1 25 µl plastic 25 nmol €185.45
1048.2 50 µl plastic 50 nmol €328.85
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