Fluorescein-12-dUTP, 25 µl, 25 nmol
Molar mass (M) 994,66 g/mol
Boiling point (bp) 100 °C
Storage temp. -20 °C
Transport temp. cooled
excl. VAT. | 25 µl per Pack Qty.
Art. No. 1048.1
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- DNAse-, RNAse-, Protease- and Phosphatase-free
- Free of PCR inhibitors like modified bases and tetra-pyrophosphate
- Adjusted to pH 7,5 for optimzed enzyme compatibility
- Highly efficient enzymatic fabrication
- Can be optimally combined with Carl ROTH ROTI®Pol DNA polymerases
PCR, cDNA synthesis, labelling and primer-extension, mutagenesis-assays, sequencing reactions and in vitro transcription.
All Carl ROTH nucleotides are manufactured from highest-quality reagents and are most thoroughly tested for quality. This testing procedure not only includes standard-PCR but also ’long range PCR’, repeated quantitative light-cycling reactions, and tests for physical stability.
Fluorescein-12-dUTP ≥95 %, 1 mM solution
Fluorescein-12-dUTP is able to replace dTTP in growing DNA-strands and is used for efficient non-radioactive DNA-labelling. Detection of labelled nucleic acids can be done by direct analysis of fluorescent signals (e.g. by fluorescence microscopy) or by incubation with AP-coupled anti-fluorescein antibodies.
Fluorescein-12-dUTP can also be used for double staining techniques in combination with rhodamine-12-dUTP or biotin-11-dUTP and corresponding antibodies or streptavidin-complexes, respectively.
Excitation: 494 nm
Emission: 520 nm
Detection of chromosome wcp21q (whole chromosome paint, red arrows) and band 11q23 (BAC, bacterial artificial chromosome, white arrows) by FISH, labelling with fluorescein-12-dUTP.
With kind permission of PD Dr. Thomas Liehr, Institute of Human Genetics and Anthropology, Friedrich-Schiller University, Jena.
Applications: Non-radioactive labelling of DNA by enzymatic reactions, e.g. PCR, reverse transcription, nick-end-translation, end-labelling or random-primed DNA-labelling. Incorporation can be done with all established DNA-polymerases (e.g. Taq-polymerase, T4 DNA-polymerase, Klenow fragment).
Testet for the lack of endo-, exodeoxyribonuclease, ribonuclease and phosphatase.
ε495 (pH 9) = 70,0 E x mmol-1 x cm-1; pH: 7,5 ±0,2
Stability testing: HPLC analysis of all four dNTPs following incubation periodes of 1–14 days at different temperatures (4 °C – green; room temperature – red; 40 °C – blue). Stability of nucleotides is >99 % even after 14 days incubation at room temperature. Even after an incubation period of 9 days at 40 °C, 85 % of all nucleotide molecules are still intact.
- Subtotal: 0.00
|Order No.||Pack Qty.||Pack.||Packaging||Price||Quantity|
|1048.1||25 µl||plastic||25 nmol||€185.45||
|1048.2||50 µl||plastic||50 nmol||€328.85||
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