Technical Data Sheet
Rhodamine-12-dUTP, 25 µl, 25 nmol
All products identified as being particularly temperaturesensitive are shipped in special ice boxes with freezer packs or in dry ice.
Ready-to-use products and reagents
Molar mass (M) 990,7 g/mol
Density (D) 1 g/cm³
Boiling point (bp) 100 °C
Storage temp. -20 °C
Transport temp. cooled
WGK 1
€235.45/Pack Qty.
excl. VAT. | 25 µl per Pack Qty.
Art. No. 1049.1
Product details
- DNAse-, RNAse- and Protease-free
- Free of PCR inhibitors like modified bases and tetra-pyrophosphate
- Adjusted to pH 7,5 for optimzed enzyme compatibility
- Highly efficient enzymatic fabrication
- Can be optimally combined with Carl ROTH ROTI®Pol DNA polymerases
All Carl ROTH nucleotides are manufactured from highest-quality reagents and are most thoroughly tested for quality. This testing procedure not only includes standard-PCR but also “long range PCR”, repeated quantitative light-cycling reactions, and tests for physical stability.
Rhodamine-12-dUTP ≥95 %, 1 mM solution
Rhodamine-12-dUTP is able to replace dTTP in growing DNA-strands and is used for efficient non-radioactive DNA-labelling. Detection of labelled nucleic acids can easily be done by direct analysis of fluorescent signals (e. g. by fluorescence microscopy).
Rhodamine-12-dUTP can also be used for double staining techniques in combination with fluorescein-12-dUTP or biotin-11-dUTP and corresponding antibodies or streptavidin-complexes, respectively.
Excitation: 505 nm
Emission: 530 nm (red)
Non-radioactive labelling of DNA by enzymatic reactions, e.g. PCR, reverse transcription, nick-end-translation, end-labelling or random-primed DNA-labelling. Incorporation can be done with all established DNA-polymerases (e.g. Taq-polymerase, T4 DNA-polymerase, Klenow fragment).
Tested for the lack of endo-, exodeoxyribonuclease, ribonuclease and phosphatase.
Rhodamine Green, mixture of 5/6 isomeres
ε505 (pH 7) = 8,5 E x mmol-1 x cm-1; pH: 7,5 ±0,2
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| Art. No. | Pack Qty. | Pack. | Packaging | Price | Quantity | |
|---|---|---|---|---|---|---|
| 1049.1 | 25 µl | plastic | 25 nmol |
€235.45 |
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| 1049.2 | 50 µl | plastic | 50 nmol |
€413.90 |
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- Subtotal: 0.00