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ROTI®Free Slide Enhancer

10x conc., BioScience Grade
Icon_DNAse-free
Icon_RNAse-free
Pack Qty.
Pack.
Storage temp. +15 to +25 °C
Transport temp. ambient temp.
WGK 2

Concentrated buffer for enhancement of detection signals in immunohistochemistry and in situ hybridization.
One bottle with 500 ml is sufficient for approx. 500 slides.

Efficient buffer for unmasking of antigens in immunohisto- and -cytochemical samples, as well as in in situ hybridizations.
Product details
Type analysis

€220.40/Pack Qty. 

excl. VAT. | 500 ml per Pack Qty.

Art. No. 9330.1

Available at short notice
from 6 Pack Qty. €209.38/Pack Qty.
from 24 Pack Qty. €198.36/Pack Qty.
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Product details


Efficient buffer for unmasking of antigens in immunohisto- and -cytochemical samples, as well as in in situ hybridizations.

  • Very effective
  • Gentle
  • Odour-free
  • Simple application
  • Suitable for formaline- and paraffin fixed samples and cryo sections

Due to the particularly adjusted formulation of the ROTI®Free Slide Enhancer Buffer, antigens and hybridization targets are unmasked very gently, but quantitatively, enhancing detection signaling significantly. Target molecules are not damaged during this process. Suitable for all common samples like formaline- and paraffin fixed tissue and cells, or cryo sections.


Directions for use

The slides are incubated in ROTI®Free Slide Enhancer-Buffer for 20-40 mins. at 70-95 °C. After repeated washing steps, the samples are assayed with antibodies or probes. The particular parameters depend on the respective samples, fixing-, and detection systems.



ROTI®Free Slide Enhancer 10x conc., BioScience Grade

9330_02
Figure 1: Fluorescence in situ hybridisation (FISH) on sections of formaline fixated, paraffin embedded tissue.
Capitals: Nuclear counter staining (Cytogreen), minuscules: Telomere-DNA FISH with red flourescence dye.

CR/cr: Detection after pretreatment with ROTI®Free Slide Enhancer.
A/a: Standard detection in 1 % triton-PBS buffer, w/o pretreatment.

With kind permission of the RIKEN Brain Science Institute (BSI), Development Research Group, Hirosawa Wako City, Saitama, Japan.

9330_03
Figure 2: Detection of proliferation by fluorescent immunohistochemical assay on tissue cryo-sections.
Capials: Detection of integrated BrdU, minuscules: Immunohistochemical PCNA detection with red flourescence dye.

CR/cr: Detection after pretreatment with ROTI®Free Slide Enhancer.
A/a: Standard detection in 1 % triton-PBS buffer, w/o pretreatment.

With kind permission of the RIKEN Brain Science Institute (BSI), Development Research Group, Hirosawa Wako City, Saitama, Japan.


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9330.1 500 ml plastic €220.40
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Certificates of Analysis

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The following analysis certificates have been found:

Type analysis

pH value (1x sol.)6,9-7,1  
RNAse≤2 ppb  
DNAse≤0,2 ppm  

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