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Elastase, 10 mg

≥200 U/mg, lyophilized
Examples of effect: Lead to damage to heath, cause irritation to eyes, skin or respiratory organs. Are fatal in larger quantities. Safety: As previously stated, in the event of skin irritation or contact with eyes, rinse with water or a suitable medium.
Examples of effect: Have an allergenic effect, carcinogenic, mutagenic, toxic to reproduction and cause developmental toxicity (reprotoxic) or damage to organs Safety: You must be well-informed before starting work with these substances; wear protective clothing and gloves, eye protection and mask or breathing protection.
Danger
H315-H319-H334-H335
i causes skin irritation, causes serious eye irritation, may cause allergy or asthma symptoms or breathing difficulties if inhaled, may cause respiratory irritation
P261 P280 P302+P352 P342+P311
i avoid breathing dust, wear protective gloves/eye protection/face protection, IF ON SKIN: Wash with plenty of water, if experiencing respiratory symptoms: Call a POISON CENTER/doctor
Pack Qty.
Pack.
Molar mass (M) ~25 900 g/mol
Storage temp. -20 °C
Transport temp. cooled
WGK 1
CAS No. 39445-21-1
EG-Nr. 254-453-6

From porcine pancreas.
1 mg/ml elastase soluble in 200 mM Tris-HCL buffer, pH 8.8.
Recommended working concentration: 1:50 to 1:100 (w/w) of elastase to substrate. pH optimum: 7.8-8.5.

Elastase is a serine protease with a broad specificity. The enzyme cleaves proteins and peptides on the carboxyl side of small hydrophobic amino acids such as Gly, Ala, Val, Leu and Ile. It also hydrolyzes amides and esters such as N-benzoyl-L-alanine methyl ester.
Product details

€91.40/Pack Qty. 

excl. VAT. | 10 mg per Pack Qty.

Art. No. 258E.1

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Product details


Elastase is a serine protease with a broad specificity. The enzyme cleaves proteins and peptides on the carboxyl side of small hydrophobic amino acids such as Gly, Ala, Val, Leu and Ile. It also hydrolyzes amides and esters such as N-benzoyl-L-alanine methyl ester.


In contrast to other proteases, elastase is able to digest native elastin, which is found in high concentrations in connective tissue. Accordingly, elastase in combination with other enzymes, such as trypsin, is often used for cell dissociation in order to separate cells from animal tissue or culture surfaces. In addition to elastin, it also hydrolyzes other proteins, including fibrin, casein, haemoglobin, albumin and denatured collagen.



Elastase ≥200 U/mg, lyophilized
Unit definition

One unit is defined as the amount of enzyme that hydrolyzes 1 μMol methoxysuccinyl-alanine-alanine-proline-valine-p-nitroanilide per minute at 37 °C and pH 7.5.



Not a medical device / Not an IVD product
Elastase
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Art. No. Pack Qty. Pack. Price Quantity
258E.1 10 mg glass

€91.40

258E.2 25 mg glass

€198.90

258E.3 100 mg glass

€666.50

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Downloads / MSDS


General information

Enzyme: a neoclassical, Greek artificial word ενζυμου, énzymon, derived from εν-, en- (in-) and ζυμη, zýmé (yeast, sourdough, archaic)
Ferments: comes from the Latin fermentum (ferments, sourdough)

There are six classes in which all enzymes are classified according to the particular reaction they catalyse:

Oxidoreductases (catalyse redox reactions)

Transferases (transfer functional groups among substrates)

Hydrolases (cleave bonds via addition of water)

Lyases/Synthases (cleave or synthesise complex products out of basic substrates without cleavage of ATP)

Isomerases (transform chemical isomers)

Ligases/Synthetases (cleave or synthesise complex products out of basic substrates via cleavage of ATP)


Enzyme: a neoclassical, Greek artificial word ενζυμου, énzymon, derived from εν-, en- (in-) and ζυμη, zýmé (yeast, sourdough, archaic)
Ferments: comes from the Latin fermentum (ferments, sourdough)

There are six classes in which all enzymes are classified according to the particular reaction they catalyse:

Oxidoreductases (catalyse redox reactions)

Transferases (transfer functional groups among substrates)

Hydrolases (cleave bonds via addition of water)

Lyases/Synthases (cleave or synthesise complex products out of basic substrates without cleavage of ATP)

Isomerases (transform chemical isomers)

Ligases/Synthetases (cleave or synthesise complex products out of basic substrates via cleavage of ATP)


Certificates of Analysis

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The following analysis certificates have been found:

Type analysis

Appearancewhite to yellowish lyophilisate
Specific activity≥200 U/mg Protein
Loss on drying≤7.0 %