Technical Data Sheet
Ligase Tth, 50 µl
All products identified as being particularly temperaturesensitive are shipped in special ice boxes with freezer packs or in dry ice.
Storage temp. -20 °C
Transport temp. cooled
WGK 1
€117.20/Pack Qty.
excl. VAT. | 50 µl per Pack Qty.
Art. No. 3736.1
Fits to / Accessories
Product details
Ligase Tth 5 U/µl, for molecular biology
Tth DNA Ligase catalyzes the NAD-dependent formation of phosphodiester bonds between adjacent 3'-hydroxyl and 5'-phosphate termini in double-stranded DNA. This ligase is inactive against single-stranded DNA or RNA and blunting DNA.
- stable at high temperatures
- ligates double-stranded DNA
- Repairs single strand breaks in double stranded DNA
The Tth DNA ligase is stable and active at much higher temperatures than conventional DNA ligases. The optimal ligation temperature range is 7-10 °C higher than that of the T4 DNA ligase and is determined by the Tm of the substrates. High ligation temperature eliminates the nonspecific ligation.
Quick Ligase
Ligation buffer (10x)
The individual components of this kit cannot be purchased separately.
The tubes included in the kit are colour coded to avoid confusion of reagents.
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| Art. No. | Pack Qty. | Pack. | Price | Quantity | |
|---|---|---|---|---|---|
| 3736.1 | 50 µl | plastic |
€117.20 |
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| 3736.2 | 500 µl | plastic |
€768.65 |
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In stock
Available
In procurement
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Delivery date currently unknown
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- Subtotal: 0.00
Downloads / MSDS
General information
Enzyme: a neoclassical, Greek artificial word ενζυμου, énzymon, derived from εν-, en- (in-) and ζυμη, zýmé (yeast, sourdough, archaic)
Ferments: comes from the Latin fermentum (ferments, sourdough)
There are six classes in which all enzymes are classified according to the particular reaction they catalyse:
• Oxidoreductases (catalyse redox reactions)
• Transferases (transfer functional groups among substrates)
• Hydrolases (cleave bonds via addition of water)
• Lyases/Synthases (cleave or synthesise complex products out of basic substrates without cleavage of ATP)
• Isomerases (transform chemical isomers)
• Ligases/Synthetases (cleave or synthesise complex products out of basic substrates via cleavage of ATP)
Certificates of Analysis
Type analysis
| Concentration | 5 U/µl |
| DNA nucleases | not detectable |