Technical Data Sheet
Proteinase K, 100 mg, 1 x 100 mg
WGK 1
CAS No. 39450-01-6
EG-Nr. 254-457-8
Art. No. 7528.3: A The stock solution (20 mg/ml) can be prepared directly in the bottle.
Highly pure serine protease for degrading proteins in biological samples. Isolated from fungi (Tritirachium album).
Proteinase K is used for the cleavage of proteins in nucleic acid preparations. It is mainly used in nucleic acid purification or for the removal of nucleases.
Proteinase K is active under a wide range of reaction conditions, including elevated temperatures and presence of SDS.
€120.30/Pack Qty.
excl. VAT. | 100 mg per Pack Qty.
Art. No. 7528.1
Product details
Proteinase K (from Tritirachium album) is a non-specific protease of the serine protease family.
Proteinase K is used for the cleavage of proteins in nucleic acid preparations. It is mainly used in nucleic acid purification or for the removal of nucleases.
Proteinase K is active under a wide range of reaction conditions, including elevated temperatures and presence of SDS.
Foreign activity: RNAse and DNAse not detectable
Optimum temperature: +65 °C.
Activity at +65 °C is ca. 12 x higher than at +25 °C. Over +65 °C, inactivation due to denaturation.
Activators: Denaturating agents like SDS (0,5-1 %), urea.
Inhibitors: Inhibition with Hg2+-ions, DFP, PMSF and phenol. Not inhibited by EDTA, sulfhydryl reagents and trypsin or chymotryps ininhibitors.
Stability: pH 4.0-12.5. pH optimum: 8,0.
Also stable even when denaturing agents, e.g. SDS and urea are present.
Stabilisers: Ca2+-ions (1-5 mM) prevent autolysis.
Proteinase K ≥30 mAnson U/mg, BioAnalysis Grade, lyophilized
A non-specific endopeptidase with strong proteolytic activity for degrading proteins in biological samples. A quality product for molecular biology with a broad scope of application.
Working solution: 50 µg/ml
Reaction buffer: 50 mM Tris-HCl; pH 7.5; 5 mM CaCl2; 0.5 % SDS
- Subtotal: 0.00
| Art. No. | Pack Qty. | Pack. | Packaging | Price | Quantity | |
|---|---|---|---|---|---|---|
| 7528.1 | 100 mg | glass | 1 x 100 mg |
€120.30 |
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| 7528.2 | 500 mg | glass | 1 x 500 mg |
€407.45 |
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| 7528.3 | 500 mg | glass | in 30 ml glass vial |
€446.15 |
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| 7528.4 | 1 g | glass | 1 x 1 g |
€671.90 |
|
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| 7528.5 | 250 mg | glass | 1 x 250 mg |
€224.70 |
|
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| 7528.6 | 5 g | glass | 1 x 5 g |
€2,682.15 |
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In stock
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- Subtotal: 0.00
Downloads / MSDS
General information
Enzyme: a neoclassical, Greek artificial word ενζυμου, énzymon, derived from εν-, en- (in-) and ζυμη, zýmé (yeast, sourdough, archaic)
Ferments: comes from the Latin fermentum (ferments, sourdough)
There are six classes in which all enzymes are classified according to the particular reaction they catalyse:
• Oxidoreductases (catalyse redox reactions)
• Transferases (transfer functional groups among substrates)
• Hydrolases (cleave bonds via addition of water)
• Lyases/Synthases (cleave or synthesise complex products out of basic substrates without cleavage of ATP)
• Isomerases (transform chemical isomers)
• Ligases/Synthetases (cleave or synthesise complex products out of basic substrates via cleavage of ATP)
Certificates of Analysis
Guarantee analysis
| Activity (Hemoglobin, 37 °C) | ≥30 U/mg |
| DNases, RNases | not detected |
| Endonucleases | not detected |
| Assay DNA | ≤10 pg/mg |
| Solubility (c=20 mg/ml in H2O) | clear, colorless |