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Ribonuclease A, 250 mg

90 U/mg (Kunitz), BioScience Grade, salt-free
Icon_DNAse-free
Icon_Protease-free
Pack Qty.
Pack.
RNase
Molar mass (M) ~13 700 g/mol
Storage temp. -20 °C
Transport temp. ambient temp.
WGK 1
CAS No. 9001-99-4
EG-Nr. 232-646-6

Acc. to Hirs, extracted from bovine pancreas.
Stock solution: 10 mg/ml in water (pH ≤6,0) or 0,01 M sodium acetate (pH 5,2). Do not heat RNase prepared in sodium acetate.

Extracted from bovine pancreas for RNA separation, especially when isolating RNA-free DNA. The enzyme cleaves RNA and produces 3' terminal nucleoside phosphates.
This quality product for molecular biology is free from salt, without proteases and is chromatographically homogeneous. Unit definition acc. to Kunitz.
Product details

€633.20/Pack Qty. 

excl. VAT. | 250 mg per Pack Qty.

Art. No. 7156.4

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Product details



Ribonuclease A 90 U/mg (Kunitz), BioScience Grade, salt-free

Extracted from bovine pancreas for RNA separation, especially when isolating RNA-free DNA. The enzyme cleaves RNA and produces 3' terminal nucleoside phosphates.
This quality product for molecular biology is free from salt, without proteases and is chromatographically homogeneous. Unit definition acc. to Kunitz.


Directions for use

Working solution: 2-10 µg/ml in 10 mM Tris (pH 7,5), 15 mM NaCl.
Note: RNase precipitates when concentrated solutions are heated/cooked at pH ≥7,0. Store solution in aliquots at -20 °C



Technical Information
Instructions for use, addition The DNase content of the RNase powder ist below detection limit. However, we nevertheless recommend pretreatment of the ribonuclease prior to using it for DNA isolation. DNase free RNase ist prepared by dissolution of the RNase powder in water, followed by cooking of the solubilised ribonuclease aliquots for 15 min. Let cool to room temperature on bench top. 
Ribonuclease A
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Art. No. Pack Qty. Pack. Price Quantity
7156.1 100 mg glass

€278.45

7156.2 500 mg glass

€1,220.15

7156.3 1 g glass

€2,338.15

7156.4 250 mg glass

€633.20

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Downloads / MSDS


General information

Enzyme: a neoclassical, Greek artificial word ενζυμου, énzymon, derived from εν-, en- (in-) and ζυμη, zýmé (yeast, sourdough, archaic)
Ferments: comes from the Latin fermentum (ferments, sourdough)

There are six classes in which all enzymes are classified according to the particular reaction they catalyse:

Oxidoreductases (catalyse redox reactions)

Transferases (transfer functional groups among substrates)

Hydrolases (cleave bonds via addition of water)

Lyases/Synthases (cleave or synthesise complex products out of basic substrates without cleavage of ATP)

Isomerases (transform chemical isomers)

Ligases/Synthetases (cleave or synthesise complex products out of basic substrates via cleavage of ATP)


Certificates of Analysis

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The following analysis certificates have been found:

Type analysis

Appearancewhite, freeze-dried powder
Assay (IEC, Amberlit CG50)>95 %
Solubility (2 mg/ml, H2O)clear, soluble
Activity (Kunitz)~90 U/mg
DNAsenot detected
Proteasenot detected