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Urease

≥220 Nessler U/mg material
Examples of effect: Lead to damage to heath, cause irritation to eyes, skin or respiratory organs. Are fatal in larger quantities. Safety: As previously stated, in the event of skin irritation or contact with eyes, rinse with water or a suitable medium.
Examples of effect: Have an allergenic effect, carcinogenic, mutagenic, toxic to reproduction and cause developmental toxicity (reprotoxic) or damage to organs Safety: You must be well-informed before starting work with these substances; wear protective clothing and gloves, eye protection and mask or breathing protection.
Danger
H315-H319-H334-H335
i causes skin irritation, causes serious eye irritation, may cause allergy or asthma symptoms or breathing difficulties if inhaled, may cause respiratory irritation
P261 P280 P304+P340 P342+P311
i avoid breathing dust, wear protective gloves/eye protection, IF INHALED: Remove person to fresh air and keep comfortable for breathing, if experiencing respiratory symptoms: Call a POISON CENTER/doctor
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Molar mass (M) ~480000 g/mol
Storage temp. -20 °C
Transport temp. cooled
WGK 1
CAS No. 9002-13-5
EG-Nr. 232-656-0

From jack beans, freeze-dried
Packaging: 0,25 Mega Unit

Hydrolase belonging to the Amidases. Metallo-enzyme. Urease hydrolasis urea, resulting in ammonia and carbon dioxide, or in ammonia- and carbonate ions, respectively. The molecule isolated from Jack beans is composed of 6 subunits of approx. 91 kDa each with an overall size of approx. 544 kDa. Nickel is the cofactor.
Using high amounts of urea as substrate (200 mM), urease is applied for visualisation of the connection between reaction rate and time. Detection is carried out via measurement of conductivity, or, photometrically, via colour change of bromothymol blue. Furthermore, model system for catalytic activation of carbon dioxide in metallo-organic chemistry.
Product details

€532.15/Pack Qty. 

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Art. No. 6065.1

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Product details


Hydrolase belonging to the Amidases. Metallo-enzyme. Urease hydrolasis urea, resulting in ammonia and carbon dioxide, or in ammonia- and carbonate ions, respectively. The molecule isolated from Jack beans is composed of 6 subunits of approx. 91 kDa each with an overall size of approx. 544 kDa. Nickel is the cofactor.
Using high amounts of urea as substrate (200 mM), urease is applied for visualisation of the connection between reaction rate and time. Detection is carried out via measurement of conductivity, or, photometrically, via colour change of bromothymol blue. Furthermore, model system for catalytic activation of carbon dioxide in metallo-organic chemistry.


Directions for use

pH optimum: 7.4.
Recommended reaction buffer system is 40 mM potassium phosphate buffer, pH 7.4. Heavy metals (e. g. lead) are inhibitory.



Urease ≥220 Nessler U/mg material
Unit definition

One unit (U) is defined as the amount of enzyme that catalyzes the liberation of 1 µmole of NH3 per min at pH 7.0 and 25 °C.



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Downloads / MSDS


General information

Enzyme: a neoclassical, Greek artificial word ενζυμου, énzymon, derived from εν-, en- (in-) and ζυμη, zýmé (yeast, sourdough, archaic)
Ferments: comes from the Latin fermentum (ferments, sourdough)

There are six classes in which all enzymes are classified according to the particular reaction they catalyse:

Oxidoreductases (catalyse redox reactions)

Transferases (transfer functional groups among substrates)

Hydrolases (cleave bonds via addition of water)

Lyases/Synthases (cleave or synthesise complex products out of basic substrates without cleavage of ATP)

Isomerases (transform chemical isomers)

Ligases/Synthetases (cleave or synthesise complex products out of basic substrates via cleavage of ATP)


Enzyme: a neoclassical, Greek artificial word ενζυμου, énzymon, derived from εν-, en- (in-) and ζυμη, zýmé (yeast, sourdough, archaic)
Ferments: comes from the Latin fermentum (ferments, sourdough)

There are six classes in which all enzymes are classified according to the particular reaction they catalyse:

Oxidoreductases (catalyse redox reactions)

Transferases (transfer functional groups among substrates)

Hydrolases (cleave bonds via addition of water)

Lyases/Synthases (cleave or synthesise complex products out of basic substrates without cleavage of ATP)

Isomerases (transform chemical isomers)

Ligases/Synthetases (cleave or synthesise complex products out of basic substrates via cleavage of ATP)


Certificates of Analysis

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The following analysis certificates have been found:

Type analysis

Appearancewhite material
Activity≥220 Nessler U/mg substance