Urease, 5 g, plastic
Density (D) 1,15 g/cm³
Storage temp. -20 °C
Transport temp. ambient temp.
CAS No. [9002-13-5]
excl. VAT. | 5 g per Pack Qty.
Art. No. 7537.1
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Urease ~1 U/mg, for biochemistry
Hydrolase belonging to the Amidases. Metallo-enzyme. Urease hydrolasis urea, resulting in ammonia and carbon dioxide, or in ammonia- and carbonate ions, respectively. The molecule isolated from Jack beans is composed of 6 subunits of approx. 91 kDa each with an overall size of approx. 544 kDa. Nickel is the cofactor. Using high amounts of urea as substrate (200 mM), urease is applied for visualisation of the connection between reaction rate and time. Detection is carried out via measurement of conductivity, or, photometrically, via colour change of bromothymol blue. Furthermore, model system for catalytic activation of carbon dioxide in metallo-organic chemistry.
1 Unit enzyme hydrolyses 1 µmol urea per minute at 25 °C (pH 8.0).
pH optimum: 7.4.
Recommended reaction buffer system is 40 mM potassium phosphate buffer, pH 7.4. Heavy metals (e.g. lead) are inhibitory.
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