Jump to content Jump to navigation menu

Urease, 5 g, plastic

~1 U/mg, for biochemistry
Examples of effect: Lead to damage to heath, cause irritation to eyes, skin or respiratory organs. Are fatal in larger quantities. Safety: As previously stated, in the event of skin irritation or contact with eyes, rinse with water or a suitable medium.
Examples of effect: Have an allergenic effect, carcinogenic, mutagenic, toxic to reproduction and cause developmental toxicity (reprotoxic) or damage to organs Safety: You must be well-informed before starting work with these substances; wear protective clothing and gloves, eye protection and mask or breathing protection.
Danger
H315-H319-H334-H335
i causes skin irritation, causes serious eye irritation, may cause allergy or asthma symptoms or breathing difficulties if inhaled, may cause respiratory irritation
P261 P280 P304+P340 P342+P311
i avoid breathing dust, wear protective gloves/eye protection, IF INHALED: Remove person to fresh air and keep comfortable for breathing, if experiencing respiratory symptoms: Call a POISON CENTER/doctor
Pack Qty.
Pack.
Molar mass (M) ~480000 g/mol
Storage temp. -20 °C
Transport temp. ambient temp.
WGK 1
CAS No. [9002-13-5]
EG-Nr. 232-656-0

From jack beans Canavalia ensiformis.

Hydrolase belonging to the Amidases. Metallo-enzyme. Urease hydrolasis urea, resulting in ammonia and carbon dioxide, or in ammonia- and carbonate ions, respectively. The molecule isolated from Jack beans is composed of 6 subunits of approx. 91 kDa each with an overall size of approx. 544 kDa. Nickel is the cofactor. Using high amounts of urea as substrate (200 mM), urease is applied for visualisation of the connection between reaction rate and time. Detection is carried out via measurement of conductivity, or, photometrically, via colour change of bromothymol blue. Furthermore, model system for catalytic activation of carbon dioxide in metallo-organic chemistry.

1 Unit enzyme hydrolyses 1 µmol urea per minute at 25 °C (pH 8.0).
Product details

€145.15/Pack Qty. 

excl. VAT. | 5 g per Pack Qty.

Art. No. 7537.1

Available at short notice
from 6 Pack Qty. €137.89/Pack Qty.
from 24 Pack Qty. €130.64/Pack Qty.
Carl Roth subscription service

Now recurring orders conveniently delivered as a subscription!

With the new Carl Roth Replenishment Service you can let products be ordered automatically which you need regularly in your lab!

How it works:
1

Put all products for your subscription in the desired quantity in the basket.

2

In the shopping cart, select the option "Order shopping cart as subscription" Order as subscription.

3

Set Starty point and interval for your subscription and submit order!

By the way: Through your account you can customize or delete your subscriptions anytime.

Product details



Urease ~1 U/mg, for biochemistry

Hydrolase belonging to the Amidases. Metallo-enzyme. Urease hydrolasis urea, resulting in ammonia and carbon dioxide, or in ammonia- and carbonate ions, respectively. The molecule isolated from Jack beans is composed of 6 subunits of approx. 91 kDa each with an overall size of approx. 544 kDa. Nickel is the cofactor. Using high amounts of urea as substrate (200 mM), urease is applied for visualisation of the connection between reaction rate and time. Detection is carried out via measurement of conductivity, or, photometrically, via colour change of bromothymol blue. Furthermore, model system for catalytic activation of carbon dioxide in metallo-organic chemistry.

1 Unit enzyme hydrolyses 1 µmol urea per minute at 25 °C (pH 8.0).


Directions for use

pH optimum: 7.4.

Recommended reaction buffer system is 40 mM potassium phosphate buffer, pH 7.4. Heavy metals (e. g. lead) are inhibitory.



Urease
Selected quantity:   0
  1. Subtotal:  0.00
Order No. Pack Qty. Pack. Price Quantity
7537.1 5 g plastic €145.15
In stock
Available at short notice
Not available
Delivery date currently unknown
Selected quantity:   0
  1. Subtotal:  0.00

Downloads / MSDS


General information

Enzyme: a neoclassical, Greek artificial word ενζυμου, énzymon, derived from εν-, en- (in-) and ζυμη, zýmé (yeast, sourdough, archaic)
Ferments: comes from the Latin fermentum (ferments, sourdough)

There are six classes in which all enzymes are classified according to the particular reaction they catalyse:

Oxidoreductases (catalyse redox reactions)

Transferases (transfer functional groups among substrates)

Hydrolases (cleave bonds via addition of water)

Lyases/Synthases (cleave or synthesise complex products out of basic substrates without cleavage of ATP)

Isomerases (transform chemical isomers)

Ligases/Synthetases (cleave or synthesise complex products out of basic substrates via cleavage of ATP)


Certificates of Analysis

You can search for and download your certificate of analysis for the selected product here. Please provide your batch number.
The following analysis certificates have been found:

Type analysis

Appearanceslightly cream-coloured powder
Activity0,8-1,2 U/mg

0 Reviews

0.0 out of five stars