Technical Data Sheet
ROTI®Prep Genomic DNA MINI, 10 preparations
Flash point (flp) 21 °C
Storage temp. +15 to +25 °C
Transport temp. ambient temp.
ADR 9 II
WGK 1
UN-Nr. 3316
Preparation of up to 65 μg gDNA from up to 40 mg tissue per column.
The isolation is carried out through the proven and reliable spin column technique, which is very easy to handle and needs only few steps.
€61.20/Pack Qty.
excl. VAT. | 1 kit per Pack Qty.
Art. No. 8472.1
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Product details
ROTI®Prep Genomic DNA MINI for molecular biology
Roti®Prep Genomic DNA MINI has been developed for the isolation of genomic DNA from various sources.
The isolation is carried out through the proven and reliable spin column technique, which is very easy to handle and needs only few steps.
- Preparation by well-known mini spin-column system
- Fast, easy and efficient
- Preparation in approx. 15 minutes (excluding lysis)
- Yield up to 65 μg gDNA, depending on source material
In addition to the especially on proteinase K adapted lysis buffer, the filtration spin column has been optimised, permitting particularly high extraction rates. Extraction from up to 40 mg tissue, therefore, allows a yield of up to μg µg DNA. The thereby extracted DNA is free of RNA and proteins, and can directly be stored or used for downstream applications.
- Tissue (up to 40 mg)
- Rodent tails (0,5-1 cm)
- FFPE tissue (formalin-fixed, paraffin-embedded)
- Buccal swabs
- Cell culture (up to 5x106 cells)
Typical test gel following isolation of genomic DNA (A) and subsequent GAPDH-PCR (B) from rodent tail (frozen, 8 mm each).
Yield of gDNA (from left to right): 22,6 μg, 27,7 μg and 28 μg. Ratio A260:A280 = 1,97±0,01.
Lysis Buffer LSA, Binding Buffer BSN, Proteinase K, Washing Buffer WST, Elution Buffer EB, spin columns, collection tubes, elution tubes, detailed instructions-for-use.
Contents of this kit may not be bought separately.
Please order ethanol 99,8 % (p.a., Art. No. 9065), RNase A (Art. No. 7156, not for FFPE tissue), and for FFPE tissue xylole (Art. No. 4436) or octane separately.
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| Art. No. | Pack Qty. | Pack. | Packaging | Price | Quantity | |
|---|---|---|---|---|---|---|
| 8472.1 | 1 kit | cardboard | 10 preparations |
€61.20 |
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| 8472.2 | 1 kit | cardboard | 50 preparations |
€203.20 |
|
|
| 8472.3 | 1 kit | cardboard | 250 preparations |
€736.40 |
|
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