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ROTI®ZOL RNA, 200 ml

ready-to-use, for molecular biology
Icon_EVE-EUD Please consider that this product might be liable to some legal sanctions as for example to obtain an end user declaration or the prohibition of some applications. For details please do contact your counterpart in our sales department!
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Icon_ready-to-use Ready-to-use products and reagents
Examples of effect: Damage metals and burn body tissues; may cause serious eye damage. Safety: Avoid contact; wear safety spectacles and gloves. In the event of contact with eyes and skin, rinse with water.
Examples of effect: Lead to damage to heath, cause irritation to eyes, skin or respiratory organs. Are fatal in larger quantities. Safety: As previously stated, in the event of skin irritation or contact with eyes, rinse with water or a suitable medium.
Examples of effect: Have an allergenic effect, carcinogenic, mutagenic, toxic to reproduction and cause developmental toxicity (reprotoxic) or damage to organs Safety: You must be well-informed before starting work with these substances; wear protective clothing and gloves, eye protection and mask or breathing protection.
Danger
H290-H302+H312+H332-H314-H341-H373-EUH032
i may be corrosive to metals, harmful if swallowed, in contact with skin or if inhaled, causes severe skin burns and eye damage, suspected of causing genetic defects, may cause damage to organs through prolonged or repeated exposure
P270 P280 P303+P361+P353 P305+P351+P338 P308+P313
i do not eat, drink or smoke when using this product, wear protective gloves/protective clothing/eye protection/face protection, IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/shower, IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing, IF exposed or concerned: Get medical advice/attention
Pack Qty.
Pack.
RNA isolation reagent
Storage temp. +4 °C
ADR 8 II
WGK 2
UN-Nr. 1760

Ready-made solution with green dye for isolation of total RNA
1 ml ROTI®ZOL RNA is used for RNA isolation from 100 mg tissue or 107 cells, respectively.

ROTI®ZOL RNA is a ready-to-use, green-coloured solution for isolating total RNA from biological samples such as cells or tissue.

ROTI®ZOL RNA is based on the phenolic isolation procedure known from Chomczynski and Sacchi (Anal. Biochem. (1987) 162:156-9). Due to the advanced and optimized formulation, ROTI®ZOL RNA results in very high recovery rates of highly pure total RNA. Application is very convenient and preparation of several samples in parallel is finished in less than one hour. Additionally, the green colour significantly simplifies the separation of the RNA containing aqueous phase, the DNA containig interphase, and the protein containing phenolic phase during the process.
Product details
Type analysis

€435.40/Pack Qty. 

excl. VAT. | 200 ml per Pack Qty.

Art. No. 9319.2

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Product details



ROTI®ZOL RNA ready-to-use, for molecular biology

ROTI®ZOL RNA is a ready-to-use, green-coloured solution for isolating total RNA from biological samples such as cells or tissue.

ROTI®ZOL RNA is based on the phenolic isolation procedure known from Chomczynski and Sacchi (Anal. Biochem. (1987) 162:156-9). Due to the advanced and optimized formulation, ROTI®ZOL RNA results in very high recovery rates of highly pure total RNA. Application is very convenient and preparation of several samples in parallel is finished in less than one hour. Additionally, the green colour significantly simplifies the separation of the RNA containing aqueous phase, the DNA containig interphase, and the protein containing phenolic phase during the process.

  • Easy and very rapid two-step RNA isolation
  • High recovery rates of intact, very pure total RNA
  • Applicable on a broad range of tissues and on cell types
  • The purified RNA is ready for use in standard downstream applications such as RT-PCR

Typical RNA yield from 5x106 cells: 30-35 μg
Typical purity of RNA prepared from 5x106 cells: 2,08 (A260/A280)

9319_02
Figures 1 and 2:
Isolation of totalRNA by Roti®ZOL RNA.
Left: Tube after centrifugation. A: Aqueous phase - RNA containig, P: Phenolic phase - protein containing. DNA accumulates in the A/P interphase.
Right: Typical gel of total RNA isolated by Roti®ZOL RNA (extracted from 5x106 HL-60 cells).


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Type analysis

Colourgreen  
pH value (25 °C)4,1-4,7  

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