Technical Data Sheet
3-Azido-7-hydroxycoumarin, 1 mg
Molar mass (M) 203,15 g/mol
Storage temp. -20 °C
Transport temp. cooled
WGK 1
CAS No. 817638-68-9
3-Azido-7-hydroxycoumarin has the special property to become fluorescent only when it is click ligated. The formed triazole expands the electron system of the dye and enables fluorescence. Therefore, there is no background signal of the click unligated leftover dye. This dye is a valuable tool for nucleic acid labeling.
€53.75/Pack Qty.
excl. VAT. | 1 mg per Pack Qty.
Art. No. 7811.1
Product details
3-Azido-7-hydroxycoumarin ≥98 %
Emissions maximum | 480 nm (after click reaction) |
Instructions for use, addition | For a 10 mM solution: add 492 µl to 1 mg. |
- Subtotal: 0.00
Art. No. | Pack Qty. | Pack. | Price | Quantity | |
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7811.1 | 1 mg | plastic |
€53.75 |
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7811.2 | 5 mg | plastic |
€145.15 |
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In stock
Available
In procurement
No longer available
Delivery date currently unknown
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- Subtotal: 0.00
Downloads / MSDS
General information
The copper(I)-catalysed azide-alkyne cycloaddition (CuAAC) is the most prominent example of a group of reactions named click reactions. According to Sharpless’ definition, these reactions are characterised by high yields, mild reaction conditions, and by their tolerance of a broad range of functional groups.[1-3] Typically, the reactions require simple or no workup or purification of the product. The most important characteristic of the CuAAC reaction is its unique bioorthogonality as neither azide nor terminal alkyne functional groups are generally present in natural systems.
References:
1 H. C. Kolb, M.G. Finn, K. B. Sharpless, Angew. Chem. Int. Ed. 2001, 40, 2004-2021.
2 C.W. Tornoe, C. Christensen, M. Meldal, J. Org. Chem. 2002, 67, 3057-3064.
3 V. V. Rostovtsev, L. G. Green, V. V. Fokin, K. B. Sharpless, Angew. Chem. 2002, 114, 2708-2711; Angew. Chem. Int. Ed. 2002, 41, 2596-2599.
In recent years, click chemistry has become increasingly important and steadily more popular in research and development. The reasons for this are not only the easy handling and the high yields but also the countless application possibilities. Carl ROTH offers you the possibility to purchase the broad portfolio of click reagents, various kits for EdU-based cell proliferation assays and numerous other applications from the well-known brand baseclick. This enables us to offer you a necessary and comprehensive assortment for click chemistry.
Fluorescent Tags
Carl ROTH offers a selection of fluorescent tags for labelling of (bio-)molecules via click chemistry. All available tags are suitable for fluorescence spectroscopy applications due to their excellent absorption properties and good solubility. Tags with PEG-spacer are beneficial for applications with as little interaction between the analyte and the tag as possible. The coumarine derivative (Art No. 7811) offers a unique feature: fluorescence is only detected after the coupling via click reaction.
Certificates of Analysis
Type analysis
Appearance | amber to brown crystalline solid |
Assay (HPLC) | ≥98 % |