5-Carboxytetramethylrhodamine Azide (5-TAMRA-Azide), 1 mg
Molar mass (M) 512,56 g/mol
Storage temp. -20 °C
Transport temp. cooled
CAS No. [1192590-89-8]
For a 10 mM solution: add 195 µl to 1 mg.
excl. VAT. | 1 mg per Pack Qty.
Art. No. 7807.1
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5-Carboxytetramethylrhodamine Azide (5-TAMRA-Azide) ≥95 %
|Emissions maximum||579 nm|
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The copper(I)-catalysed azide-alkyne cycloaddition (CuAAC) is the most prominent example of a group of reactions named click reactions. According to Sharpless’ definition, these reactions are characterised by high yields, mild reaction conditions, and by their tolerance of a broad range of functional groups.[1-3] Typically, the reactions require simple or no workup or purification of the product. The most important characteristic of the CuAAC reaction is its unique bioorthogonality as neither azide nor terminal alkyne functional groups are generally present in natural systems.
1 H. C. Kolb, M.G. Finn, K. B. Sharpless, Angew. Chem. Int. Ed. 2001, 40, 2004-2021.
2 C.W. Tornoe, C. Christensen, M. Meldal, J. Org. Chem. 2002, 67, 3057-3064.
3 V. V. Rostovtsev, L. G. Green, V. V. Fokin, K. B. Sharpless, Angew. Chem. 2002, 114, 2708-2711; Angew. Chem. Int. Ed. 2002, 41, 2596-2599.
Carl ROTH offers a selection of fluorescent tags for labelling of (bio-)molecules via click chemistry. All available tags are suitable for fluorescence spectroscopy applications due to their excellent absorption properties and good solubility. Tags with PEG-spacer are beneficial for applications with as little interaction between the analyte and the tag as possible. The coumarine derivative (Art No. 7811) offers a unique feature: fluorescence is only detected after the coupling via click reaction.
|Appearance||dark red solid|
|Assay (LCMS)||≥95 %|
|Absorption max. (DMF)||546 nm|
|Emission max. (DMF)||579 nm|
|Molar extinction coefficient ε||91 000 cm -1M-1|
|Solubility||DMSO, DMF, MeOH|