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6-Carboxyfluorescein Azide (6-FAM-Azide), 10 mg

From  baseclick
≥95 %
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4-((3-Azidopropyl)carbamoyl)-2-(6-hydroxy-3-oxo-3H-xanthen-9-yl)benzoic acid, 6-FAM-Azide
Empirical formula C24H18N4O6
Molar mass (M) 458,43 g/mol
Storage temp. -20 °C
Transport temp. cooled
WGK 1
CAS No. 1386385-76-7

FITC - Fluorescent dye to label DNA/RNA
Fluorescein Azide (6-FAM-Azide) is a fluorescent dye that allows efficient labeling of biomolecules by click reactions. With identical spectral properties to FITC (Fluorescein-5,6-isothiocyanate), ATTO488, Alexa Fluor 488 or DyLight 488 and alike, it is well suited for most fluorescent detection methods.

€123.65/Pack Qty. 

excl. VAT. | 10 mg per Pack Qty.

Art. No. 7806.3

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Product details



6-Carboxyfluorescein Azide (6-FAM-Azide) ≥95 %

Technical Information
Emissions maximum 516 nm 
6-Carboxyfluorescein Azide (6-FAM-Azide)
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Art. No. Pack Qty. Pack. Price Quantity
7806.1 1 mg plastic

€48.40

7806.2 5 mg plastic

€86.00

7806.3 10 mg plastic

€123.65

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Downloads / MSDS

General information

Click Chemistry

The copper(I)-catalysed azide-alkyne cycloaddition (CuAAC) is the most prominent example of a group of reactions named click reactions. According to Sharpless’ definition, these reactions are characterised by high yields, mild reaction conditions, and by their tolerance of a broad range of functional groups.[1-3] Typically, the reactions require simple or no workup or purification of the product. The most important characteristic of the CuAAC reaction is its unique bioorthogonality as neither azide nor terminal alkyne functional groups are generally present in natural systems.


References:
1 H. C. Kolb, M.G. Finn, K. B. Sharpless, Angew. Chem. Int. Ed. 2001, 40, 2004-2021.
2 C.W. Tornoe, C. Christensen, M. Meldal, J. Org. Chem. 2002, 67, 3057-3064.
3 V. V. Rostovtsev, L. G. Green, V. V. Fokin, K. B. Sharpless, Angew. Chem. 2002, 114, 2708-2711; Angew. Chem. Int. Ed. 2002, 41, 2596-2599.


Click Chemistry

In recent years, click chemistry has become increasingly important and steadily more popular in research and development. The reasons for this are not only the easy handling and the high yields but also the countless application possibilities. Carl ROTH offers you the possibility to purchase the broad portfolio of click reagents, various kits for EdU-based cell proliferation assays and numerous other applications from the well-known brand baseclick. This enables us to offer you a necessary and comprehensive assortment for click chemistry.


Tags

Fluorescent Tags

Carl ROTH offers a selection of fluorescent tags for labelling of (bio-)molecules via click chemistry. All available tags are suitable for fluorescence spectroscopy applications due to their excellent absorption properties and good solubility. Tags with PEG-spacer are beneficial for applications with as little interaction between the analyte and the tag as possible. The coumarine derivative (Art No. 7811) offers a unique feature: fluorescence is only detected after the coupling via click reaction.


Certificates of Analysis

You can search for and download your certificate of analysis for the selected product here. Please provide your batch number.
The following analysis certificates have been found:

Type analysis

Appearanceyellow to orange solid
Assay (HPLC)≥95 %